A fatal case of louping-ill in a dog: immunolocalization and full genome sequencing of the virus

Dagleish, M.P., Clark, J.J., Robson, C., Tucker, M., Orton, R.J. and Rocchi, M.S. (2018) A fatal case of louping-ill in a dog: immunolocalization and full genome sequencing of the virus. Journal of Comparative Pathology, 165, pp. 23-32. (doi:10.1016/j.jcpa.2018.09.004) (PMID:30502792) (PMCID:PMC6302148)

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Abstract

Louping-ill (LI), caused by louping-ill virus (LIV), results in a frequently fatal encephalitis primarily affecting sheep and red grouse (Lagopus lagopus scotica), but it does occur in other species. An adult male Border collie dog was definitively diagnosed with fatal LI and the lesion profile, LIV antigen distribution and full genome sequence of the LIV responsible were investigated to determine if this differed significantly from sheep-derived LIV. No gross lesions were present. The histological lesions were confined to the central nervous system and comprised of lymphocytic perivascular cuffs, glial foci, neuronal necrosis and neuronophagia. Immunolocalization of viral antigen showed small amounts present in neurons only. These histological and immunohistochemical findings were similar to those reported in affected sheep. Compared with published full genome sequences of sheep-derived LIV, only very minor differences were present and phylogenetically the virus clustered individually between a subclade containing Scottish strains, LIV 369/T2 and G and another subclade containing an English isolate LIV A. The LIV isolated from the dog shares a common progenitor with LIV A. These findings suggest there is no canine-specific LIV strain, dogs are susceptible to sheep-associated strains of LI and with the increase in tick prevalence, and therefore exposure to LIV, a safe, effective vaccine for dogs may be required.

Item Type:Articles
Additional Information:MR and MPD are funded by the Scottish Government. JC was supported by the BBSRC Doctoral Training Partnership 2012, (grant number BB/J013854/1) as well as the UK Medical Research Council (MC_UU_12014/8). RJO was supported by the UK Medical Research Council (grant number MC_UU_12014/12).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Clark, Mr Jordan and Rocchi, Dr Mara and Orton, Dr Richard
Authors: Dagleish, M.P., Clark, J.J., Robson, C., Tucker, M., Orton, R.J., and Rocchi, M.S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Comparative Pathology
Publisher:Elsevier
ISSN:0021-9975
ISSN (Online):1532-3129
Published Online:19 October 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Journal of Comparative Pathology 165:23-32
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656551Arbovirus interactions with arthropod hostsAlain KohlMedical Research Council (MRC)MC_UU_12014/8MVLS III - CENTRE FOR VIRUS RESEARCH