Diagnosis of non-effusive feline infectious peritonitis by reverse transcriptase quantitative PCR from mesenteric lymph node fine-needle aspirates

Dunbar, D. et al. (2018) Diagnosis of non-effusive feline infectious peritonitis by reverse transcriptase quantitative PCR from mesenteric lymph node fine-needle aspirates. Journal of Feline Medicine and Surgery, (doi:10.1177/1098612X18809165) (PMID:30407137)

Dunbar, D. et al. (2018) Diagnosis of non-effusive feline infectious peritonitis by reverse transcriptase quantitative PCR from mesenteric lymph node fine-needle aspirates. Journal of Feline Medicine and Surgery, (doi:10.1177/1098612X18809165) (PMID:30407137)

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Abstract

The aim of this study was to evaluate a feline coronavirus (FCoV) reverse transcriptase quantitative PCR (RT-qPCR) on fine-needle aspirates (FNAs) from mesenteric lymph nodes (MLNs) collected in sterile saline for the purpose of diagnosing non-effusive feline infectious peritonitis (FIP) in cats. First, the ability of the assay to detect viral RNA in MLN FNA preparations compared with MLN biopsy preparations was assessed in matched samples from eight cats. Second, a panel of MLN FNA samples was collected from a series of cats representing non-effusive FIP cases (n = 20), FCoV-seropositive individuals (n = 8) and FCoV seronegative individuals (n = 18). Disease status of the animals was determined using a combination of gross pathology, histopathology and/or 'FIP profile', consisting of serology, clinical pathology and clinical signs. Viral RNA was detected in 18/20 non-effusive FIP cases; it was not detected in two cases that presented with neurological FIP. Samples from 18 seronegative non-FIP control cats and 7/8 samples from seropositive non-FIP control cats contained no detectable viral RNA. Thus, as a method for diagnosing non-effusive FIP, MLN FNA RT-qPCR had an overall sensitivity of 90.0% and specificity of 96.1%. In cases with a high index of suspicion of disease, RT-qPCR targeting FCoV in MLN FNA can provide important information to support the ante-mortem diagnosis of non-effusive FIP. Importantly, viral RNA can be reliably detected in MLN FNA samples in saline submitted via the national mail service. When applied in combination with biochemistry, haematology and serological tests in cases with a high index of suspicion of disease the results of this assay may be used to support a diagnosis of non-effusive FIP.

Item Type:Articles
Additional Information:The Angelica Trust funds FIP research at the University of Glasgow. The John Robertson Bequest (University of Glasgow) and the BBSRC REP funded the studentship of Wendy Kwok.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McDonald, Mr Michael and Dunbar, Mrs Dawn and Addie, Dr Diane and Johnston, Dr Pamela and Irvine, Mr Richard and Weir, Dr William and Nicolson, Dr Lesley and Graham, Dr Elizabeth
Authors: Dunbar, D., Kwok, W., Graham, E., Armitage, A., Irvine, R., Johnston, P., McDonald, M., Montgomery, D., Nicolson, L., Robertson, E., Weir, W., and Addie, D. D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Journal of Feline Medicine and Surgery
Publisher:Elsevier
ISSN:1098-612X
ISSN (Online):1532-2750
Published Online:08 November 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Journal of Feline Medicine and Surgery 2018
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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