Astrocytes express Mxi2, a splice isoform of p38MAPK

Bodega, G., Ciordia, S., Suárez, I., López-Fernández, L. A., Vacas, E., Tejeda, G. S. , Albert, M. A., Juárez, S., Albar, J. P. and Fernández, B. (2009) Astrocytes express Mxi2, a splice isoform of p38MAPK. Journal of Molecular Histology, 40(5-6), pp. 325-329. (doi: 10.1007/s10735-009-9248-8) (PMID:20043236)

Abstract

Mitogen-activated protein kinases (MAPKs) are a superfamily of cytoplasmic serine/threonine kinases that transduce many types of extracellular stimuli into cellular responses. p38MAPK is a member of this family with its active form in a diphosphorylated state (p38MAPKdiP). Two strong anti-p38MAPKdiP immunoreactive bands (apparent molecular weight 38 and 34 kDa) were detected by Western blotting in cultured astrocytes. Using a specific antibody and employing immunoprecipitation procedures and SELDI-TOF analysis, the 34 kDa band was found to correspond to Mxi2, a splice variant of p38MAPK; cultured astrocytes therefore express Mxi2. Separate protein extractions of different subcellular fractions, and fluorescent immunovisualisation employing confocal microscopy, showed Mxi2 to have a non-nuclear, cytosolic distribution in the studied cells. ERK1/2, protein whose intracellular distribution is influenced by Mxi2, showed the same cytoplasmic pattern than Mxi2.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Tejeda, Dr Gonzalo
• Authors: Bodega, G., Ciordia, S., Suárez, I., López-Fernández, L. A., Vacas, E., Tejeda, G. S., Albert, M. A., Juárez, S., Albar, J. P., and Fernández, B.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Journal of Molecular Histology
• Publisher: Springer
• ISSN: 1567-2379
• ISSN (Online): 1567-2387
• Published Online: 31 December 2009