Integrated dataset on acute phase protein response in chicken challenged with Escherichia coli lipopolysaccharide endotoxin

Horvatić, A., Guillemin, N., Kaab, H., McKeegan, D. , O'Reilly, E., Bain, M. , Kuleš, J. and Eckersall, P. D. (2018) Integrated dataset on acute phase protein response in chicken challenged with Escherichia coli lipopolysaccharide endotoxin. Data In Brief, 21, pp. 684-699. (doi: 10.1016/j.dib.2018.09.103)

Abstract

Data herein describe the quantitative changes in the plasma proteome in chickens challenged with lipopolysaccharide (LPS), a bacterial endotoxin known to stimulate the host innate immune system obtained by shotgun quantitative proteomic tandem mass tags approach using high-resolution Orbitrap technology. Statistical and bioinformatic analyses were performed to specify the effect of bacterial endotoxin. Plasma from chicken (N=6) challenged with Escherichia coli (LPS) (2 mg/kg body weight) was collected pre (0 h) and at 12, 24, 48, and 72 h post injection along with plasma from a control group (N=6) challenged with sterile saline. Protein identification and relative quantification were performed using Proteome Discoverer, and data were analysed using R. Gene Ontology terms were analysed by the Cytoscape application ClueGO based on Gallus gallus GO Biological Process database, and refined by REVIGO. Absolute quantification of several acute phase proteins, e.g. alpha-1-acid glycoprotein (AGP), serum amyloid A (SAA) and ovotrensferrin (OVT) was performed by immunoassays to validate the LC-MS results. The data contained within this article are directly related to our research article”Quantitative proteomics using tandem mass tags in relation to the acute phase protein response in chicken challenged with Escherichia coli lipopolysaccharide endotoxin” [1]. The raw mass spectrometric data generated in this study were deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD009399 (http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD009399).

• Item Type: Articles
• Additional Information: The authors acknowledge the European Commission for funding the ERA chair team VetMedZg (ERA Chair Initiative).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Eckersall, Professor David and O'Reilly, Dr Emily and McKeegan, Dr Dorothy and Bain, Professor Maureen and KAAB, Haider
• Authors: Horvatić, A., Guillemin, N., Kaab, H., McKeegan, D., O'Reilly, E., Bain, M., Kuleš, J., and Eckersall, P. D.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Data In Brief
• Publisher: Elsevier
• ISSN: 2352-3409
• ISSN (Online): 2352-3409
• Published Online: 17 October 2018
• Copyright Holders: Copyright © 2018 Elsevier
• First Published: First published in Data In Brief 21: 684-699
• Publisher Policy: Reproduced under a Creative Commons License