Impact of frequency of denture cleaning on microbial and clinical parameters - a bench to chairside approach

Ramage, G. et al. (2019) Impact of frequency of denture cleaning on microbial and clinical parameters - a bench to chairside approach. Journal of Oral Microbiology, 11(1), 1538437. (doi:10.1080/20002297.2018.1538437) (PMID:30598732) (PMCID:PMC6225516)

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Abstract

Objective: Robust scientific and clinical evidence of how to appropriately manage denture plaque is lacking. This two-part study (i) developed an in vitro model of denture plaque removal, and (ii) assessed effectiveness of these approaches in a randomised clinical trial. Method: (i) a complex denture plaque model was developed using the dominant microbial genera from a recent microbiome analyses. Biofilms formed on polymethylmethacrylate were brushed daily with a wet toothbrush, then either treated daily for 5 days or only on Days 1 and 5 with Polident® denture cleanser tablets (3 min soaking). Quantitative and qualitative microbiological assessments were performed. (ii), an examiner-blind, randomised, crossover study of complete maxillary denture wearers was performed (n = 19). Either once-daily for 7 days or on Day 7 only, participants soaked dentures for 15 min using Corega® denture cleansing tables, then brushed. Denture plaque microbiological assessment used sterilized filter paper discs. Results: The in vitro model showed daily cleaning with denture cleanser plus brushing significantly reduced microbial numbers compared to intermittent denture cleaning with daily brushing (p < 0.001). The clinical component of the study showed a statistically significant reduction in denture plaque microbial numbers in favour of daily versus weekly treatment (aerobic bacteria p = 0.0144). Both in vitro and in vivo studies showed that denture plaque biofilm composition were affected by different treatment arms. Conclusions: This study demonstrated that daily denture cleansing regimens are superior to intermittent denture cleansing, and that cleansing regimens can induce denture plaque compositional changes. Clinicaltrials.gov registration: NCT02780661.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cross, Dr Laura and Jose, Mr Anto and Bagg, Professor Jeremy and Macinnes, Mr Andrew and Culshaw, Professor Shauna and Robertson, Mr Douglas and Czesnikiewicz-Guzik, Dr Marta and Ramage, Professor Gordon and Brown, Mrs Clare and Sherry, Dr Leighann and McKenzie, Mrs Debbie and O'Donnell, Dr Lindsay
Authors: Ramage, G., O'Donnell, L., Sherry, L., Culshaw, S., Bagg, J., Czesnikiewicz-Guzik, M., Brown, C., McKenzie, D., Cross, L., Macinnes, A., Bradshaw, D., Varghese, R., Pereira, P. G., Jose, A., Sanyal, S., and Robertson, D.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Journal of Oral Microbiology
Publisher:Taylor & Francis
ISSN:2000-2297
ISSN (Online):2000-2297
Published Online:29 October 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Journal of Oral Microbiology 11(1):1538437
Publisher Policy:Reproduced under a Creative Commons License

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