Impact of frequency of denture cleaning on microbial and clinical parameters - a bench to chairside approach

Ramage, G. et al. (2019) Impact of frequency of denture cleaning on microbial and clinical parameters - a bench to chairside approach. Journal of Oral Microbiology, 11(1), 1538437. (doi: 10.1080/20002297.2018.1538437) (PMID:30598732) (PMCID:PMC6225516)

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Abstract

Objective: Robust scientific and clinical evidence of how to appropriately manage denture plaque is lacking. This two-part study (i) developed an in vitro model of denture plaque removal, and (ii) assessed effectiveness of these approaches in a randomised clinical trial. Method: (i) a complex denture plaque model was developed using the dominant microbial genera from a recent microbiome analyses. Biofilms formed on polymethylmethacrylate were brushed daily with a wet toothbrush, then either treated daily for 5 days or only on Days 1 and 5 with Polident® denture cleanser tablets (3 min soaking). Quantitative and qualitative microbiological assessments were performed. (ii), an examiner-blind, randomised, crossover study of complete maxillary denture wearers was performed (n = 19). Either once-daily for 7 days or on Day 7 only, participants soaked dentures for 15 min using Corega® denture cleansing tables, then brushed. Denture plaque microbiological assessment used sterilized filter paper discs. Results: The in vitro model showed daily cleaning with denture cleanser plus brushing significantly reduced microbial numbers compared to intermittent denture cleaning with daily brushing (p < 0.001). The clinical component of the study showed a statistically significant reduction in denture plaque microbial numbers in favour of daily versus weekly treatment (aerobic bacteria p = 0.0144). Both in vitro and in vivo studies showed that denture plaque biofilm composition were affected by different treatment arms. Conclusions: This study demonstrated that daily denture cleansing regimens are superior to intermittent denture cleansing, and that cleansing regimens can induce denture plaque compositional changes. Clinicaltrials.gov registration: NCT02780661.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cross, Dr Laura and Macinnes, Mr Andrew and Czesnikiewicz-Guzik, Dr Marta and Brown, Mrs Clare and Bagg, Professor Jeremy and Sherry, Dr Leighann and Jose, Mr Anto and Ramage, Professor Gordon and Culshaw, Professor Shauna and Robertson, Mr Douglas and McKenzie, Mrs Debbie and O'Donnell, Dr Lindsay
Authors: Ramage, G., O'Donnell, L., Sherry, L., Culshaw, S., Bagg, J., Czesnikiewicz-Guzik, M., Brown, C., McKenzie, D., Cross, L., Macinnes, A., Bradshaw, D., Varghese, R., Pereira, P. G., Jose, A., Sanyal, S., and Robertson, D.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Journal of Oral Microbiology
Publisher:Taylor & Francis
ISSN:2000-2297
ISSN (Online):2000-2297
Published Online:29 October 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Journal of Oral Microbiology 11(1):1538437
Publisher Policy:Reproduced under a Creative Commons License

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