In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy

Noonan, J. et al. (2018) In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy. Theranostics, 8(22), pp. 6195-6209. (doi:10.7150/thno.28665) (PMID:30613292) (PMCID:PMC6299693)

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Abstract

Vascular immune-inflammatory responses play a crucial role in the progression and outcome of atherosclerosis. The ability to assess localized inflammation through detection of specific vascular inflammatory biomarkers would significantly improve cardiovascular risk assessment and management; however, no multi-parameter molecular imaging technologies have been established to date. Here, we report the targeted in vivo imaging of multiple vascular biomarkers using antibody-functionalized nanoparticles and surface-enhanced Raman scattering (SERS). Methods: A series of antibody-functionalized gold nanoprobes (BFNP) were designed containing unique Raman signals in order to detect intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and P-selectin using SERS. Results: SERS and BFNP were utilized to detect, discriminate and quantify ICAM-1, VCAM-1 and P-selectin in vitro on human endothelial cells and ex vivo in human coronary arteries. Ultimately, non-invasive multiplex imaging of adhesion molecules in a humanized mouse model was demonstrated in vivo following intravenous injection of the nanoprobes. Conclusion: This study demonstrates that multiplexed SERS-based molecular imaging can indicate the status of vascular inflammation in vivo and gives promise for SERS as a clinical imaging technique for cardiovascular disease in the future.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McInnes, Professor Iain and Maffia, Dr Pasquale and Asiala, Dr Steven and Bradshaw, Dr Angela and Graham, Professor Duncan and Guzik, Professor Tomasz and Noonan, Dr Jonathan and Garside, Professor Paul and Grassia, Dr Gianluca and Brewer, Professor James and Sattar, Professor Naveed and Scales, Dr Hannah and MacRitchie, Mr Neil and Meehan, Dr Gavin
Authors: Noonan, J., Asiala, S. M., Grassia, G., MacRitchie, N., Gracie, K., Carson, J., Moores, M., Girolami, M., Bradshaw, A. C., Guzik, T. J., Meehan, G. R., Scales, H. E., Brewer, J. M., McInnes, I. B., Sattar, N., Faulds, K., Garside, P., Graham, D., and Maffia, P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Science and Engineering > School of Chemistry
Journal Name:Theranostics
Publisher:Ivyspring International Publisher
ISSN:1838-7640
ISSN (Online):1838-7640
Published Online:29 November 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Theranostics 8(22): 6195-6209
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
644661In Situ Nanoparticle Assemblies for Healthcare Diagnostics and TherapyPasquale MaffiaEngineering and Physical Sciences Research Council (EPSRC)EP/L014165/1III -IMMUNOLOGY
682541NanoMATePasquale MaffiaEuropean Commission (EC)661369III -IMMUNOLOGY
617771BHF centre of excellenceRhian TouyzBritish Heart Foundation (BHF)RE/13/5/30177RI CARDIOVASCULAR & MEDICAL SCIENCES
525072Cytoadherence-mediated pathology in cerebral malariaJames BrewerWellcome Trust (WELLCOTR)095507/Z/11/AIII -IMMUNOLOGY