Multi-purpose SLM-light-sheet microscope

Garbellotto, C. and Taylor, J. M. (2018) Multi-purpose SLM-light-sheet microscope. Biomedical Optics Express, 9(11), pp. 5419-5436. (doi:10.1364/BOE.9.005419)

Garbellotto, C. and Taylor, J. M. (2018) Multi-purpose SLM-light-sheet microscope. Biomedical Optics Express, 9(11), pp. 5419-5436. (doi:10.1364/BOE.9.005419)

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Abstract

By integrating a phase-only Spatial Light Modulator (SLM) into the illumination arm of a cylindrical-lens-based Selective Plane Illumination Microscope (SPIM), we have created a versatile system able to deliver high quality images by operating in a wide variety of different imaging modalities. When placed in a Fourier plane, the SLM permits modulation of the microscope’s light-sheet to implement imaging techniques such as structured illumination, tiling, pivoting, autofocusing and pencil beam scanning. Previous publications on dedicated microscope setups have shown how these techniques can deliver improved image quality by rejecting out-of-focus light (structured illumination and pencil beam scanning), reducing shadowing (light-sheet pivoting), and obtaining a more uniform illumination by moving the highest-resolution region of the light-sheet across the imaging Field of View (tiling). Our SLM-SPIM configuration is easy to build and use, and has been designed to allow all of these techniques to be employed on an easily reconfigurable optical setup, compatible with the OpenSPIM design. It offers the possibility to choose between three different light-sheets, in thickness and height, which can be selected according to the characteristics of the sample and the imaging technique to be applied. We demonstrate the flexibility and performance of the system with results obtained by applying a variety of different imaging techniques on samples of fluorescent beads, zebrafish embryos, and optically cleared whole mouse brain samples. Thus our approach allows easy implementation of advanced imaging techniques while retaining the simplicity of a cylindrical-lens-based light-sheet microscope.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Taylor, Dr Jonathan and Garbellotto, Chiara
Authors: Garbellotto, C., and Taylor, J. M.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Biomedical Optics Express
Publisher:Optical Society of America
ISSN:2156-7085
ISSN (Online):2156-7085
Published Online:12 October 2018
Copyright Holders:Copyright © 2018 The Optical Society
First Published:First published in Biomedical Optics Express 9(11): 5419-5436
Publisher Policy:Reproduced under a Creative Commons License
Related URLs:
Data DOI:10.5525/gla.researchdata.648

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
636381EPSRC Centre for Doctoral Training in Sensing and MeasurementAndrew HarveyEngineering and Physical Sciences Research Council (EPSRC)EP/L016753/1SCHOOL OF PHYSICS & ASTRONOMY