Chromosomal copy number variation analysis by next generation sequencing confirms ploidy stability in Trypanosoma brucei subspecies

Almeida, L. V., Coqueiro-Dos-Santos, A., Rodriguez-Luiz, G. F., McCulloch, R. , Bartholomeu, D. C. and Reis-Cunha, J. L. (2018) Chromosomal copy number variation analysis by next generation sequencing confirms ploidy stability in Trypanosoma brucei subspecies. Microbial Genomics, 4(10), e000223. (doi:10.1099/mgen.0.000223) (PMID:30256189) (PMCID:PMC6249438)

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Abstract

Although aneuploidy usually results in severe abnormalities in multicellular eukaryotes, recent data suggest that it could be beneficial for unicellular eukaryotes, such as yeast and trypanosomatid parasites, providing increased survival under stressful conditions. Among characterized trypanosomatids, Trypanosoma cruzi, Trypanosoma brucei and species from the genus Leishmania stand out due to their importance in public health, infecting around 20 million people worldwide. The presence of aneuploidies in T. cruzi and Leishmania was recently confirmed by analysis based on next generation sequencing (NGS) and fluorescence in situ hybridization, where they have been associated with adaptation during transmission between their insect vectors and mammalian hosts and in promoting drug resistance. Although chromosomal copy number variations (CCNVs) are present in the aforementioned species, PFGE and fluorescence cytophotometry analyses suggest that aneuploidies are absent from T. brucei. A re-evaluation of CCNV in T. b gambiense based on NGS reads confirmed the absence of aneuploidies in this subspecies. However, the presence of aneuploidies in the other two T. brucei subspecies, T. b. brucei and T. b. rhodesiense, has not been evaluated using NGS approaches. In the present work, we tested for aneuploidies in 26 T. brucei isolates, including samples from the three T. brucei subspecies, by both allele frequency and read depth coverage analyses. These analyses showed that none of the T. brucei subspecies presents aneuploidies, which could be related to differences in the mechanisms of DNA replication and recombination in these parasites when compared with Leishmania.

Item Type:Articles
Additional Information:Work in RM’s lab is supported by the BBSRC [BB/K006495/1, BB/M028909/1, BB/N016165/1], the European Commission [RECREPEMLE] and the Wellcome Trust [206815/Z/17/Z]. The Wellcome Centre for Molecular Parasitology, Glasgow, is supported by core funding from the Wellcome Trust [104111]. DCB’s group is supported by Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Instituto Nacional de Ciência e Tecnologia de Vacinas (INCTV) – Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). DCB is a CNPq research fellow. JLRC and LVA received scholarships from CNPq, and GFRL and ACS received scholarships from CAPES.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McCulloch, Professor Richard
Authors: Almeida, L. V., Coqueiro-Dos-Santos, A., Rodriguez-Luiz, G. F., McCulloch, R., Bartholomeu, D. C., and Reis-Cunha, J. L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Microbial Genomics
Publisher:Microbiology Society
ISSN:2057-5858
ISSN (Online):2057-5858
Published Online:27 September 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Microbial Genomics 4(10):e000223
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
170547The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/ZRIII - Parasitology