Biomimetic oyster shell-replicated topography alters the behaviour of human skeletal stem cells

Waddell, S. J., de Andrés, M. C., Tsimbouri, P. M. , Alakpa, E. V., Cusack, M., Dalby, M. J. and Oreffo, R. O.C. (2018) Biomimetic oyster shell-replicated topography alters the behaviour of human skeletal stem cells. Journal of Tissue Engineering, 9, p. 2041731418794007. (doi:10.1177/2041731418794007) (PMID:30202512) (PMCID:PMC6124183)

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Abstract

The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers (  < 0.05) and (  < 0.01) and increased metabolite intensity (  < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity (  < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype.

Item Type:Articles
Additional Information:The work in the authors’ laboratories was supported by grants from the BBSRC (BB/L021072/1 and BB/L00609X/1 and UK Regenerative Medicine Platform (MR/K026682/1) and University of Southampton to R.O.C.O. M.J.D. and M.C. acknowledge MRC grant MR/K011278/1.
Keywords:Nacre, bone regeneration, osteogenic differentiation, skeletal stem cell, topography.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tsimbouri, Dr Penelope and Dalby, Professor Matthew
Authors: Waddell, S. J., de Andrés, M. C., Tsimbouri, P. M., Alakpa, E. V., Cusack, M., Dalby, M. J., and Oreffo, R. O.C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Tissue Engineering
Publisher:SAGE Publications
ISSN:2041-7314
ISSN (Online):2041-7314
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Journal of Tissue Engineering 9: 2041731418794007
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
609651Stem Cells metabolomics for bone therapies and tissue engineering.Maggie CusackMedical Research Council (MRC)MR/K011278/1SCHOOL OF GEOGRAPHICAL & EARTH SCIENCES