Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells

Sinjab, F., Awuah, D., Gibson, G. , Padgett, M. , Ghaemmaghami, A. M. and Notingher, I. (2018) Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells. Optics Express, 26(19), pp. 25211-25225. (doi: 10.1364/OE.26.025211)

Abstract

We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of spontaneous Raman spectroscopy from trapped objects. Raman scattering from each laser focus is spatially filtered using reflective apertures on a digital micro-mirror device, which can be reconfigured with flexible patterns at video rate. We discuss operation of the instrument, and performance and viability considerations for biological measurements. We then demonstrate the capability of the instrument for fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems.

• Item Type: Articles
• Additional Information: This work was supported by the Engineering and Physical Sciences Research Council [grant number EP/L025620/1, EP/M506588/1].
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Gibson, Dr Graham and Padgett, Professor Miles
• Authors: Sinjab, F., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., and Notingher, I.
• College/School: College of Science and Engineering > School of Physics and Astronomy
• Journal Name: Optics Express
• Publisher: Optical Society of America
• ISSN: 1094-4087
• ISSN (Online): 1094-4087
• Published Online: 12 September 2018
• Copyright Holders: Copyright © 2018 Optical Society of America
• First Published: First published in Optics Express 26(19): 25211-25225
• Publisher Policy: Reproduced under a Creative Commons License