Ho, K. L., Gabrielsen, M., Beh, P. L., Kueh, C. L., Qiu Xian, T., Streetley, J. , Tan, W. S. and Bhella, D. (2018) Structure of the Macrobrachium rosenbergii nodavirus: a new genus within the Nodaviridae? PLoS Biology, 16(10), e3000038. (doi: 10.1371/journal.pbio.3000038) (PMID:30346944) (PMCID:PMC6211762)
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Abstract
Macrobrachium rosenbergii nodavirus (MrNV) is a pathogen of freshwater prawns that poses a threat to food security and causes significant economic losses in the aquaculture industries of many developing nations. A detailed understanding of the MrNV virion structure will inform the development of strategies to control outbreaks. The MrNV capsid has also been engineered to display heterologous antigens, and thus knowledge of its atomic resolution structure will benefit efforts to develop tools based on this platform. Here, we present an atomic-resolution model of the MrNV capsid protein (CP), calculated by cryogenic electron microscopy (cryoEM) of MrNV virus-like particles (VLPs) produced in insect cells, and three-dimensional (3D) image reconstruction at 3.3 Å resolution. CryoEM of MrNV virions purified from infected freshwater prawn post-larvae yielded a 6.6 Å resolution structure, confirming the biological relevance of the VLP structure. Our data revealed that unlike other known nodavirus structures, which have been shown to assemble capsids having trimeric spikes, MrNV assembles a T = 3 capsid with dimeric spikes. We also found a number of surprising similarities between the MrNV capsid structure and that of the Tombusviridae: 1) an extensive network of N-terminal arms (NTAs) lines the capsid interior, forming long-range interactions to lace together asymmetric units; 2) the capsid shell is stabilised by 3 pairs of Ca2+ ions in each asymmetric unit; 3) the protruding spike domain exhibits a very similar fold to that seen in the spikes of the tombusviruses. These structural similarities raise questions concerning the taxonomic classification of MrNV.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ho, Dr Kok Lian and Beh, Poay Ling and Streetley, Dr James and Bhella, Professor David and Gabrielsen, Dr Mads |
| Creator Roles: | Ho, K. L.Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Writing – original draft, Writing – review and editing Gabrielsen, M.Formal analysis, Writing – review and editing Beh, P. L.Formal analysis, Investigation Streetley, J.Formal analysis, Investigation Bhella, D.Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review and editing |
| Authors: | Ho, K. L., Gabrielsen, M., Beh, P. L., Kueh, C. L., Qiu Xian, T., Streetley, J., Tan, W. S., and Bhella, D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | PLoS Biology |
| Publisher: | Public Library of Science |
| ISSN: | 1544-9173 |
| ISSN (Online): | 1545-7885 |
| Copyright Holders: | Copyright © 2018 Ho et al. |
| First Published: | First published in PLoS Biology 16(10): 3000038 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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