Fast, Image-based Autofocus System for High-resolution Optical Microscopy of Whole Mouse Brains

Silvestri, L., Müllenbroich, M. C. , Costantini, I., Di Giovanna, A. P., Sacconi, L. and Pavone, F. S. (2017) Fast, Image-based Autofocus System for High-resolution Optical Microscopy of Whole Mouse Brains. Bio-Optics Design and Application 2017, San Diego, CA., 2-5 April 2017. JTu4A.8. ISBN 9781943580255 (doi:10.1364/BODA.2017.JTu4A.8)

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Abstract

We describe an image-based method for single-shot measurement and real-time correction of defocus in an optical microscope. The method is validated both in bright-field, epifluorescence and light-sheet microscopy.

Item Type:Conference or Workshop Item
Additional Information:OSA Technical Digest (online) (Optical Society of America, 2017), paper JTu4A.8.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Muellenbroich, Dr Caroline
Authors: Silvestri, L., Müllenbroich, M. C., Costantini, I., Di Giovanna, A. P., Sacconi, L., and Pavone, F. S.
College/School:College of Science and Engineering > School of Physics and Astronomy
ISBN:9781943580255

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