Silvestri, L., Müllenbroich, M. C. , Costantini, I., Di Giovanna, A. P., Sacconi, L. and Pavone, F. S. (2017) Fast, Image-based Autofocus System for High-resolution Optical Microscopy of Whole Mouse Brains. Bio-Optics Design and Application 2017, San Diego, CA., 2-5 April 2017. JTu4A.8. ISBN 9781943580255 (doi: 10.1364/BODA.2017.JTu4A.8)
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Abstract
We describe an image-based method for single-shot measurement and real-time correction of defocus in an optical microscope. The method is validated both in bright-field, epifluorescence and light-sheet microscopy.
Item Type: | Conference or Workshop Item |
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Additional Information: | OSA Technical Digest (online) (Optical Society of America, 2017), paper JTu4A.8. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Muellenbroich, Dr Caroline |
Authors: | Silvestri, L., Müllenbroich, M. C., Costantini, I., Di Giovanna, A. P., Sacconi, L., and Pavone, F. S. |
College/School: | College of Science and Engineering > School of Physics and Astronomy |
ISBN: | 9781943580255 |
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