Identification of the growth arrest and DNA damage protein GADD34 in the normal human heart and demonstration of alterations in expression following myocardial ischaemia

Morton, E., Macrae, I.M., McCabe, C., Brown, S.M. and White, F. (2006) Identification of the growth arrest and DNA damage protein GADD34 in the normal human heart and demonstration of alterations in expression following myocardial ischaemia. International Journal of Cardiology, 107(1), pp. 126-129. (doi: 10.1016/j.ijcard.2005.01.051)

Abstract

Growth arrest and DNA damage protein 34 (GADD34) is a multifunctional protein upregulated in response to cellular stress and is believed to mediate DNA repair and restore protein synthesis. In the present study we have examined GADD34 immunoreactivity in human myocardial tissue at defined survival times following cardiac arrest and determined alterations in expression following ischaemia. In the normal human heart, GADD34 immunoreactivity was generally intense and present within most cells. GADD34 immunoreactivity was downregulated in tissue displaying ischaemic damage and remained intense in adjacent non-infarcted tissue. Unlike brain, GADD34 was not found to be upregulated in the peri-infarct zone. Cells displaying apoptotic changes were located in regions displaying reduced GADD34 immunoreactivity. In the brain, it is thought that GADD34 supports re-initiation of protein synthesis following ischaemia. Similarly, GADD34 may perform important functions in cardiac tissue in response to ischaemia.

• Item Type: Articles
• Additional Information: NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Cardiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Cardiology, [107, 1, (2006)] DOI: 10.1016/j.ijcard.2005.01.051
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Macrae, Professor Mhairi and McCabe, Dr Chris
• Authors: Morton, E., Macrae, I.M., McCabe, C., Brown, S.M., and White, F.
• Subjects: R Medicine > R Medicine (General)
• College/School: College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
• Journal Name: International Journal of Cardiology
• Publisher: Elsevier
• ISSN: 0167-5273
• ISSN (Online): 1874-1754
• Published Online: 22 April 2005
• Copyright Holders: Copyright © 2006 Elsevier
• First Published: First published in International Journal of Cardiology 107(1):126-129
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher