An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites

Patil, S. B. , Dheeman, D. S., Al-Rawhani, M. A., Velugotla, S. , Nagy, B., Cheah, B. C. , Grant, J. P. , Accarino, C., Barrett, M. P. and Cumming, D. R.S. (2018) An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites. Biosensors and Bioelectronics, 122, pp. 88-94. (doi:10.1016/j.bios.2018.09.013) (PMID:30245326)

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Abstract

Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously will underpin a new generation of precision diagnostic tools. Here, we report the development of a handheld device based on complementary metal oxide semiconductor (CMOS) technology with multiple isolated micro-well reaction zones and integrated optical sensing allowing simultaneous enzyme-based assays of multiple metabolites (choline, xanthine, sarcosine and cholesterol) associated with multiple diseases. These metabolites were measured in clinically relevant concentration range with minimum concentrations measured: 25 μM for choline, 100 μM for xanthine, 1.25 μM for sarcosine and 50 μM for cholesterol. Linking the device to an Android-based user interface allows for quantification of metabolites in serum and urine within 2 min of applying samples to the device. The quantitative performance of the device was validated by comparison to accredited tests for cholesterol and glucose.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cumming, Professor David and Al-Rawhani, Dr Mohammed and Accarino, Claudio and Barrett, Professor Michael and Patil, Dr Samadhan and Cheah, Dr Boon Chong and Nagy, Mr Bence and Dheeman, Dr Dharmendra and Velugotla, Dr Srinivas and Grant, Dr James
Authors: Patil, S. B., Dheeman, D. S., Al-Rawhani, M. A., Velugotla, S., Nagy, B., Cheah, B. C., Grant, J. P., Accarino, C., Barrett, M. P., and Cumming, D. R.S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:Biosensors and Bioelectronics
Publisher:Elsevier
ISSN:0956-5663
ISSN (Online):1873-4235
Published Online:05 September 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Biosensors and Bioelectronics 122:88-94
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
605821The Multi-Corder: Poly-Sensor TechnologyDavid CummingEngineering and Physical Sciences Research Council (EPSRC)EP/K021966/1ENG - ENGINEERING ELECTRONICS & NANO ENG