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Structural and functional conservation at the boundaries of the chicken β-globin domain

Saitoh, N., Bell, A.C., Recillas-Targa, F., West, A.G., Simpson, M., Pikaart, M., and Felsenfeld, G. (2000) Structural and functional conservation at the boundaries of the chicken β-globin domain. EMBO Journal, 19 (10). pp. 2315-2322. ISSN 0261-4189 (doi:10.1093/emboj/19.10.2315)

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Abstract

We show that the 3′ boundary of the chicken β-globin locus bears striking structural similarities to the 5′ boundary. In erythroid cells a clear transition in DNase I sensitivity of chromatin at the 3′ end of the locus is observed, the location of this transition is marked by a constitutive DNase I hypersensitive site (HS), and DNA spanning this site has the enhancer-blocking capacity of an insulator. This HS contains a binding site for the transcription factor CTCF. As in the case of the 5′ insulator, the CTCF site is both necessary and sufficient for the enhancer-blocking activity of the 3′ boundary. The position of this insulator is consistent with our proposal that it may function to maintain the distinct regulatory programs of the globin genes and their closely appended 3′ neighbor, an odorant receptor gene. We conclude that both boundaries of the chicken β-globin domain are capable of playing functionally similar roles and that the same protein is a necessary component of the molecular mechanism through which these boundaries are defined.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:West, Dr Adam
Authors: Saitoh, N., Bell, A.C., Recillas-Targa, F., West, A.G., Simpson, M., Pikaart, M., and Felsenfeld, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:EMBO Journal
ISSN:0261-4189

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