Quasispecies changes with distinctive point mutations in the Hepatitis C virus internal ribosome entry site (IRES) derived from PBMCs and plasma

Mercuri, L., Thomson, E. C. , Hughes, J. and Karayiannis, P. (2018) Quasispecies changes with distinctive point mutations in the Hepatitis C virus internal ribosome entry site (IRES) derived from PBMCs and plasma. Advances in Virology, 2018, 4835252. (doi:10.1155/2018/4835252) (PMID:30581467) (PMCID:PMC6276526)

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Abstract

The 5’ untranslated region (UTR) of the hepatitis C virus (HCV) genome contains the internal ribosome entry site (IRES), a highly conserved RNA structure essential for cap-independent translation of the viral polyprotein. HCV, apart from the liver, is thought to be associated with lymphocyte subpopulations of peripheral blood mononuclear cells (PBMCs), in lymph nodes and brain tissue. In this study, RT-PCR, cloning, and sequence analysis were employed to investigate the quasispecies nature of the 5’UTR following extraction of viral RNA from PBMCs and plasma of HCV infected individuals. The nucleotide variation between IRES-derived sequences from PBMCs and plasma indicated the existence of polymorphic sites within the IRES. HCV isolates had divergent variants with unique mutations particularly at positions 107, 204, and 243 of the IRES. Most of the PBMC-derived sequences contained an A-A-A variant at these positions. The mutations associated with the IRESes suggested the presence of unique quasispecies populations in PBMCs compared with plasma.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Thomson, Professor Emma and Hughes, Dr Joseph
Authors: Mercuri, L., Thomson, E. C., Hughes, J., and Karayiannis, P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Advances in Virology
Publisher:Hindawi
ISSN:1687-8639
ISSN (Online):1687-8647
Published Online:19 November 2018
Copyright Holders:Copyright © 2018 Luca Mercuri et al.
First Published:First published in Advances in Virology 2018:4835252
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
645101T-cell mediated evolution of hepatitis C virus during acute infectionEmma ThomsonWellcome Trust (WELLCOTR)102789/Z/13/ZMVLS III - CENTRE FOR VIRUS RESEARCH