Blobs and curves: object-based colocalisation for plant cells

Nelson, C. , Duckney, P., Hawkins, T. J., Deeks, M. J., Laissue, P. P., Hussey, P. J. and Boguslaw, O. (2014) Blobs and curves: object-based colocalisation for plant cells. Functional Plant Biology, 42(5), pp. 471-485. (doi:10.1071/FP14047)

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Abstract

Blobs and curves occur everywhere in plant bioimaging: from signals of fluorescence-labelled proteins, through cytoskeletal structures, nuclei staining and cell extensions such as root hairs. Here we look at the problem of colocalisation of blobs with blobs (protein-protein colocalisation) and blobs with curves (organelle-cytoskeleton colocalisation). This article demonstrates a clear quantitative alternative to pixel-based colocalisation methods and, using object-based methods, can quantify not only the level of colocalisation but also the distance between objects. Included in this report are computational algorithms, biological experiments and guidance for those looking to increase their use of computationally-based and quantified analysis of bioimages.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Nelson, Dr Chas
Authors: Nelson, C., Duckney, P., Hawkins, T. J., Deeks, M. J., Laissue, P. P., Hussey, P. J., and Boguslaw, O.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Functional Plant Biology
Publisher:CSIRO Publishing
ISSN:1445-4408
ISSN (Online):1445-4416

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