Removing physiological motion from intravital and clinical functional imaging data

Warren, S. C. et al. (2018) Removing physiological motion from intravital and clinical functional imaging data. eLife, 7, e35800. (doi: 10.7554/elife.35800) (PMID:29985127) (PMCID:PMC6037484)

Abstract

Intravital microscopy can provide unique insights into the function of biological processes in a native context. However, physiological motion caused by peristalsis, respiration and the heartbeat can present a significant challenge, particularly for functional readouts such as fluorescence lifetime imaging (FLIM), which require longer acquisition times to obtain a quantitative readout. Here, we present and benchmark Galene, a versatile multi-platform software tool for image-based correction of sample motion blurring in both time resolved and conventional laser scanning fluorescence microscopy data in two and three dimensions. We show that Galene is able to resolve intravital FLIM-FRET images of intra-abdominal organs in murine models and NADH autofluorescence of human dermal tissue imaging subject to a wide range of physiological motions. Thus, Galene can enable FLIM imaging in situations where a stable imaging platform is not always possible and rescue previously discarded quantitative imaging data.

• Item Type: Articles
• Keywords: Tools and resources, cell biology, computational and systems biology, FLIM, FRET, motion correction, intravital microscopy, multiphoton, human, mouse.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Anderson, Professor Kurt and Morton, Professor Jen and Strathdee, Mr Douglas

Creator Roles

Morton, J.: Resources, Writing – review and editing
Strathdee, D.: Resources, Writing – review and editing
Anderson, K.: Resources, Writing – review and editing

• Authors: Warren, S. C., Nobis, M., Magenau, A., Mohammed, Y. H., Herrmann, D., Moran, I., Vennin, C., Conway, J. R.W., Mélénec, P., Cox, T. R., Wang, Y., Morton, J. P., Welch, H. C.E., Strathdee, D., Anderson, K. I., Phan, T. G., Roberts, M. S., and Timpson, P.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: eLife
• Publisher: eLife Sciences Publications
• ISSN: 2050-084X
• ISSN (Online): 2050-084X
• Copyright Holders: Copyright © 2018 Warren et al.
• First Published: First published in eLife 7:e35800
• Publisher Policy: Reproduced under a Creative Commons License