A hybrid paper-based microfluidic platform toward veterinary P-ELISA

Busin, V., Burgess, S. and Shu, W. (2018) A hybrid paper-based microfluidic platform toward veterinary P-ELISA. Sensors and Actuators B: Chemical, 273, pp. 536-542. (doi:10.1016/j.snb.2018.06.075)

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Abstract

This study describes a novel fabrication method to produce a hybrid paper-based microfluidic platform, termed Multi-Pad Paper Plate (MP3) for transfer and optimisation of enzyme-linked immunoassays (ELISA) on paper (P-ELISA). The new fabrication technique is based on a combination of laser micromachining of paper and packaging through thermal lamination. This method is simple, rapid and of high-resolution and can produce robust, versatile and low-cost devices, compatible with a standard 96-well microplate format. The MP3 was used to transfer a sandwich ELISA for detection of bovine haptoglobin (Hp), a marker of inflammation in animals allowing optimisation of the assay in the new format. Using the MP3, a standard curve was generated and the limit of detection achieved was 0.73 μg/ml. The optimised protocol was also applied to the detection of Hp in bovine serum, demonstrating the possibility of using this platform with biological samples. The new platform allowed for a reduction of 93% in time and of 88% in cost for performing the assay and represents a valid alternative to other commonly used device fabrication methods, especially in the context of low-resource settings. It has the potential to translate similar assays into P-ELISA and offers a starting point for achieving animal-side testing.

Item Type:Articles
Additional Information:The authors would like to thank Moredun Scientific Ltd. and Heriot-Watt University for funding this research.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Busin, Valentina
Authors: Busin, V., Burgess, S., and Shu, W.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Sensors and Actuators B: Chemical
Publisher:Elsevier
ISSN:0925-4005
ISSN (Online):1873-3077
Published Online:18 June 2018
Copyright Holders:Copyright © 2018 Elsevier B.V.
First Published:First published in Sensors and Actuators B: Chemical 273: 536-542
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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