Three-dimensional super-resolution correlation-differential confocal microscopy with nanometer axial focusing accuracy

Zhao, W., Sun, Y., Wang, Y., Qiu, L., Shao, R. and Cui, H. (2018) Three-dimensional super-resolution correlation-differential confocal microscopy with nanometer axial focusing accuracy. Optics Express, 26(12), pp. 15759-15768. (doi:10.1364/OE.26.015759)

Zhao, W., Sun, Y., Wang, Y., Qiu, L., Shao, R. and Cui, H. (2018) Three-dimensional super-resolution correlation-differential confocal microscopy with nanometer axial focusing accuracy. Optics Express, 26(12), pp. 15759-15768. (doi:10.1364/OE.26.015759)

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Abstract

We present a correlation-differential confocal microscopy (CDCM), a novel method that can simultaneously improve the three-dimensional spatial resolution and axial focusing accuracy of confocal microscopy (CM). CDCM divides the CM imaging light path into two paths, where the detectors are before and after the focus with an equal axial offset in opposite directions. Then, the light intensity signals received from the two paths are processed by the correlation product and differential subtraction to improve the CM spatial resolution and axial focusing accuracy, respectively. Theoretical analyses and preliminary experiments indicate that, for the excitation wavelength of λ = 405 nm, numerical aperture of NA = 0.95, and the normalized axial offset of uM = 5.21, the CDCM resolution is improved by more than 20% and more than 30% in the lateral and axial directions, respectively, compared with that of the CM. Also, the axial focusing resolution important for the imaging of sample surface profiles is improved to 1 nm.

Item Type:Articles
Additional Information:National Natural Science Foundation of China (NSFC) (61635003, 61475020,51535002) and National Key R&D Program of China (2016YFF0201005).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cui, Dr Han
Authors: Zhao, W., Sun, Y., Wang, Y., Qiu, L., Shao, R., and Cui, H.
Subjects:Q Science > QC Physics
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Research Group:OSA
Journal Name:Optics Express
Publisher:Optical Society of America
ISSN:1094-4087
ISSN (Online):1094-4087
Copyright Holders:Copyright © 2018 The Optical Society of America under the terms of the OSA Open Access Publishing Agreement
First Published:First published in Optical Express 26(12):15759-15768
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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