Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies

Costa, E., González-García, C., Gómez Ribelles, J. L. and Salmeron-Sanchez, M. (2018) Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies. Journal of Tissue Engineering, 9, pp. 1-10. (doi: 10.1177/2041731418789829) (PMID:30093985) (PMCID:PMC6080075)

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Articular chondrocytes are difficult to grow as they lose their characteristic phenotype following expansion on standard tissue culture plates. Here we show that culturing them on surfaces of poly(L-lactic acid), PLLA, of well-defined microtopography allows expansion and maintenance of characteristic chondrogenic markers. We investigated the dynamics of human chondrocyte dedifferentiation on the different PLLA microtopograhies by the expression of collagen type I, collagen type II and aggrecan at different culture times. When seeded on PLLA, chondrocytes maintained their characteristic hyaline phenotype up to 7 days, which allowed expand the initial cell population approximately six times without cell dedifferentiation. Maintenance of cell phenotype was afterwards correlated to cell adhesion on the different substrates. Chondrocytes adhesion occurs via the a5b1 integrin on PLLA, suggesting cellfibronectin interactions. However, a2b1 integrin is mainly expressed on the control substrate after 1 day of culture and the characteristic chondrocytic markers are lost (collagen type II expression is overcome by the synthesis of collagen type I). Expanding chondrocytes on PLLA might be an effective solution to prevent dedifferentiation and improving the number of cells needed for autologous chondrocyte transplantation.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel
Authors: Costa, E., González-García, C., Gómez Ribelles, J. L., and Salmeron-Sanchez, M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Tissue Engineering
ISSN (Online):2041-7314
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Journal of Tissue Engineering 9: 2041731418789829
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
722061Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - BIOMEDICAL ENGINEERING