Gating control and K+ uptake by the KAT1 K+ channel leaveraged through membrane anchoring of the trafficking protein SYP121

Lefoulon, C., Waghmare, S., Karnik, R. and Blatt, M. R. (2018) Gating control and K+ uptake by the KAT1 K+ channel leaveraged through membrane anchoring of the trafficking protein SYP121. Plant, Cell and Environment, 41(11), pp. 2668-2677. (doi:10.1111/pce.13392) (PMID:29940699) (PMCID:PMC6220998)

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Abstract

Vesicle traffic is tightly coordinated with ion transport for plant cell expansion through physical interactions between subsets of vesicle‐trafficking (so‐called SNARE) proteins and plasma membrane Kv channels, including the archetypal inward‐rectifying K+ channel, KAT1 of Arabidopsis. Ion channels open and close rapidly over milliseconds, whereas vesicle fusion events require many seconds. Binding has been mapped to conserved motifs of both the Kv channels and the SNAREs, but knowledge of the temporal kinetics of their interactions, especially as it might relate to channel gating and its coordination with vesicle fusion remains unclear. Here we report that the SNARE SYP121 promotes KAT1 gating through a persistent interaction that alters the stability of the channel, both in its open and closed states. We show, too, that SYP121 action on the channel open state requires SNARE anchoring in the plasma membrane. Our findings indicate that SNARE binding confers a conformational bias that encompasses the microscopic kinetics of channel gating, with leverage applied through the SNARE anchor in favor of the open channel.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Karnik, Dr Rucha and Blatt, Professor Michael and Lefoulon, Dr Cecile and Waghmare, Dr Sakharam
Authors: Lefoulon, C., Waghmare, S., Karnik, R., and Blatt, M. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant, Cell and Environment
Publisher:Wiley
ISSN:0140-7791
ISSN (Online):1365-3040
Published Online:25 June 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Plant, Cell and Environment 41(11): 2668-2677
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
626121Stomatal-based systems analysis of water use efficiencyMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/L001276/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
563753MAGIC - A Multi-tiered approach to generating increased carbon dioxide in the chloroplastMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/M01133X/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
656891Analysing GORK clustering for enhanced stomatal controlMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/M001601/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
703441Dissecting a new and vital checkpoint in SNARE recycling and plant growthMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/N006909/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
650621Developing a synthetic approach to manipulating guard cell membrane transport and stomatal controlMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/L019205/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
696911NEURICEMichael BlattEuropean Commission (EC)678168RI MOLECULAR CELL & SYSTEMS BIOLOGY