Enrichment of innate lymphoid cell populations in gingival tissue

Brown, J.L. , Campbell, L., Malcolm, J. , Adrados Planell, A., Butcher, J.P. and Culshaw, S. (2018) Enrichment of innate lymphoid cell populations in gingival tissue. Journal of Dental Research, 97(12), pp. 1399-1405. (doi:10.1177/0022034518782141) (PMID:29928824)

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Abstract

Innate lymphoid cells (ILCs) are a population of lymphocytes that act as the first line of immunologic defense at mucosal surfaces. The ILC family in the skin, lungs, and gastrointestinal tissues has been investigated, and there are reports of individual subsets of ILCs in the oral tissues. We sought to investigate the whole ILC population (group 1, 2, and 3 subsets) in the murine gingivae and the lymph nodes draining the oral cavity. We show that ILCs made up a greater proportion of the whole CD45+ lymphocyte population in the murine gingivae (0.356% ± 0.039%) as compared with the proportion of ILCs in the draining lymph nodes (0.158% ± 0.005%). Cytokine profiling of the ILC populations demonstrated different proportions of ILC subsets in the murine gingivae versus the regional lymph nodes. The majority of ILCs in the draining lymph nodes expressed IL-5, whereas there were equal proportions of IFN-γ- and IL-5 expressing ILCs in the oral mucosa. The percentage of IL-17+ ILCs was comparable between the murine gingivae and the oral draining lymph nodes. These data suggest an enrichment of ILCs in the murine gingivae, and these ILCs reflect a cytokine profile discrepant to that of the local draining lymph nodes. These studies indicate diversity and enrichment of ILCs at the oral mucosal surface. The function of ILCs in the oral cavity remains to be determined; here, we provide a premise of ILC populations that merits future consideration in investigations of mouse models and human tissues.

Item Type:Articles
Additional Information:A. Adrados Planell and this work were also funded by the Marie Curie ITN (RAPID 290246).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Butcher, Mr John and Malcolm, Dr Jennifer and Campbell, Lauren Dee and Brown, Mr Jason and Culshaw, Professor Shauna and Adrados Planell, Ana
Authors: Brown, J.L., Campbell, L., Malcolm, J., Adrados Planell, A., Butcher, J.P., and Culshaw, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Journal of Dental Research
Publisher:SAGE Publications
ISSN:0022-0345
ISSN (Online):1544-0591
Published Online:21 June 2018
Copyright Holders:Copyright © 2018 International and American Associations for Dental Research
First Published:First published in Journal of Dental Research 97(12): 1399-1405
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
535611Gums and JointsShauna CulshawEuropean Commission (EC)261460SM - DENTAL SCHOOL
640761T cells and teeth - what do oral mucosal T cells do in health and disease?Shauna CulshawSir Jules Thorn Charitable Trust (JTHORNTR)13/JTPhDSM - DENTAL SCHOOL
656691Investigating the immune response to citrullinated antigenShauna CulshawArthritis Research UK (ARTRESUK)20823SM - DENTAL SCHOOL