Community and functional shifts in ammonia oxidizers across terrestrial and marine (soil/sediment) boundaries in two coastal Bay ecosystems

Zhang, L., Duff, A. and Smith, C. J. (2018) Community and functional shifts in ammonia oxidizers across terrestrial and marine (soil/sediment) boundaries in two coastal Bay ecosystems. Environmental Microbiology, 20(8), pp. 2834-2853. (doi:10.1111/1462-2920.14238) (PMID:29687546)

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Abstract

Terrestrial–marine boundaries are significant sites of biogeochemical activity with delineated gradients from land to sea. While niche differentiation of ammonia‐oxidizing archaea (AOA) and bacteria (AOB) driven by pH and nitrogen is well known, the patterns and environmental drivers of AOA and AOB community structure and activity across soil‐sediment boundaries have not yet been determined. In this study, nitrification potential rate, community composition and transcriptional activity of AOA and AOB in soil, soil/sediment interface and sediments of two coastal Bays were characterized using a combination of field investigations and microcosm incubations. At DNA level, amoA gene abundances of AOA were significantly greater than AOB in soil, while in sediments AOB were significantly more abundant than AOA, but at the soil/sediment interface there were equal numbers of AOA and AOB amoA genes. Microcosm incubations provided further evidence, through qPCR and DGGE‐sequencing analysis of amoA transcripts, that AOA were active in soil, AOB in sediment and both AOA and AOB were active at the soil/sediment interface. The AOA and AOB community composition shifted across the coastal soil‐interface‐sediment gradient with salinity and pH identified as major environmental drivers.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Duff, Dr Aoife and Smith, Dr Cindy
Authors: Zhang, L., Duff, A., and Smith, C. J.
College/School:College of Science and Engineering > School of Engineering > Infrastructure and Environment
Journal Name:Environmental Microbiology
Publisher:Wiley
ISSN:1462-2912
ISSN (Online):1462-2920
Published Online:24 April 2018
Copyright Holders:Copyright © 2018 Society for Applied Microbiology and John Wiley and Sons Ltd
First Published:First published in Environmental Microbiology 20(8): 2834-2853
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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