RAB40C regulates RACK1 stability via the ubiquitin-proteasome system

Daly, J. P., Whiteley, E., Freeley, M., Long, A., Malacrida, B., Kiely, P. and Baillie, G. S. (2018) RAB40C regulates RACK1 stability via the ubiquitin-proteasome system. Future Science OA, 4(7), FSO317. (doi:10.4155/fsoa-2018-0022) (PMID:30112187) (PMCID:PMC6088270)

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Abstract

Aim: RACK1 is a multifunctional scaffolding protein that is expressed in many cellular compartments, orchestrating a number of signaling processes. RACK1 acts as a signaling hub to localize active enzymes to discrete locations; therefore tight control of RACK1 is vital to cellular homeostasis. Our aim was to identify the mechanisms responsible for RACK1 turnover and show that degradation is directed by the ubiquitin proteasome system. Results: Using siRNA screening, we identified RAB40C as the ubiquitin E3 ligase responsible for ubiquitination of RACK1, and that the action of RAB40C in controlling RACK1 levels is crucial to both cancer cell growth and migration of T cells. Conclusion: Our data suggest that manipulation of RACK1 levels in this way may provide a novel strategy to explore RACK1 function.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Baillie, Professor George and WHITELEY, Ellanor
Authors: Daly, J. P., Whiteley, E., Freeley, M., Long, A., Malacrida, B., Kiely, P., and Baillie, G. S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Future Science OA
Publisher:Future Science
ISSN:2056-5623
ISSN (Online):2056-5623
Published Online:02 July 2018
Copyright Holders:Copyright © 2018 Glasgow University
First Published:First published in Future Science OA 4(7): FSO317
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
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