Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon

Ranford-Cartwright, L.C. , Taylor, J., Umasunthar, T., Taylor, L.H., Babiker, H.A., Lell, B., Schmidt-Ott, J.R., Lehman, L.G., Walliker, D. and Kremsner, P.G. (1997) Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon. Transactions of the Royal Society of Tropical Medicine and Hygiene, 91(6), pp. 719-724. (doi:10.1016/s0035-9203(97)90539-3) (PMID:9509189)

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Abstract

Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a posttreatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ranford-Cartwright, Dr Lisa
Authors: Ranford-Cartwright, L.C., Taylor, J., Umasunthar, T., Taylor, L.H., Babiker, H.A., Lell, B., Schmidt-Ott, J.R., Lehman, L.G., Walliker, D., and Kremsner, P.G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Transactions of the Royal Society of Tropical Medicine and Hygiene
Publisher:Elsevier
ISSN:0035-9203
ISSN (Online):1878-3503

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