Genetic hybrids of Plasmodium falciparum identified by amplification of genomic DNA from single oocysts

Ranford-Cartwright, L. C. , Balfe, P., Carter, R. and Walliker, D. (1991) Genetic hybrids of Plasmodium falciparum identified by amplification of genomic DNA from single oocysts. Molecular and Biochemical Parasitology, 49(2), pp. 239-243. (doi: 10.1016/0166-6851(91)90067-G) (PMID:1775167)

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Abstract

Individual oocysts from Plasmodium falciparum-infected Anopheles gambiae and Anopheles stephensi mosquitoes have been examined by the PCR technique, after their removal from the midgut. The DNA obtained from these oocysts has been amplified using oligonucleotide primers specific for part of the merozoite surface antigen MSA-1 gene. This technique distinguishes oocysts which are the products of self-fertilisation events from those which are the products of cross-fertilisation between different parasite clones.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ranford-Cartwright, Dr Lisa
Authors: Ranford-Cartwright, L. C., Balfe, P., Carter, R., and Walliker, D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Molecular and Biochemical Parasitology
Publisher:Elsevier
ISSN:0166-6851
ISSN (Online):1872-9428

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