Community development between Porphyromonas gingivalis and Candida albicans mediated by InlJ and Als3

Sztukowska, M. N., Dutton, L. C., Delaney, C., Ramsdale, M., Ramage, G. , Jenkinson, H. F., Nobbs, A. H. and Lamont, R. J. (2018) Community development between Porphyromonas gingivalis and Candida albicans mediated by InlJ and Als3. mBio, 9(2), e00202-18. (doi:10.1128/mBio.00202-18) (PMID:29691333) (PMCID:PMC5915736)

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Abstract

The pleiomorphic yeast Candida albicans is a significant pathogen in immunocompromised individuals. In the oral cavity, C. albicans is an inhabitant of polymicrobial communities, and interspecies interactions promote hyphal formation and biofilm formation. C. albicans colonizes the subgingival area, and the frequency of colonization increases in periodontal disease. In this study, we investigated the interactions between C. albicans and the periodontal pathogen Porphyromonas gingivalis. C. albicans and P. gingivalis were found to coadhere in both the planktonic and sessile phases. Loss of the internalin-family protein InlJ abrogated adhesion of P. gingivalis to C. albicans, and recombinant InlJ protein competitively inhibited interspecies binding. A mutant of C. albicans deficient in expression of major hyphal protein Als3 showed diminished binding to P. gingivalis, and InlJ interacted with Als3 heterologously expressed in Saccharomyces cerevisiae. Transcriptional profiling by RNA sequencing (RNA-Seq) established that 57 genes were uniquely upregulated in an InlJ-dependent manner in P. gingivalis-C. albicans communities, with overrepresentation of those corresponding to 31 gene ontology terms, including those associated with growth and division. Of potential relevance to the disease process, C. albicans induced upregulation of components of the type IX secretion apparatus. Collectively, these findings indicate that InlJ-Als3-dependent binding facilitates interdomain community development between C. albicans and P. gingivalis and that P. gingivalis has the potential for increased virulence within such communities.

Item Type:Articles
Keywords:Candida albicans, Porphyromonas gingivalis, RNA-Seq, microbial communities, polymicrobial synergy
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Delaney, Christopher and Ramage, Professor Gordon
Authors: Sztukowska, M. N., Dutton, L. C., Delaney, C., Ramsdale, M., Ramage, G., Jenkinson, H. F., Nobbs, A. H., and Lamont, R. J.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:mBio
Publisher:American Society for Microbiology
ISSN:2150-7511
ISSN (Online):2150-7511
Published Online:24 April 2018
Copyright Holders:Copyright © 2018 Sztukowska et al.
First Published:First published in mBio 9(2):e00202-18
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
744671Understanding processes and mechanisms affecting the oral microbiome using OMICs approachesGordon RamageBiotechnology and Biological Sciences Research Council (BBSRC)BB/P504567/1SM - DENTAL SCHOOL