Arabidopsis blue light receptor phototropin 1 undergoes blue light-induced activation in membrane microdomains

Xue, Y. et al. (2018) Arabidopsis blue light receptor phototropin 1 undergoes blue light-induced activation in membrane microdomains. Molecular Plant, 11(6), pp. 846-859. (doi:10.1016/j.molp.2018.04.003) (PMID:29689384)

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Abstract

Phototropin (phot)-mediated signaling initiated by blue light (BL) plays a critical role in optimizing photosynthetic light capture at the plasma membrane (PM) in plants. However, the mechanisms underlying the regulation of phot activity at the PM in response to BL remain largely unclear. In this study, by single-particle tracking and step-wise photobleaching analysis we demonstrated that in the dark phot1-GFP proteins remain in an inactive state and mostly present as a monomer. The phot1-GFP diffusion rate and its dimerization increased in a dose-dependent manner in response to BL. In contrast, BL did not affect the lateral diffusion of kinase-inactive phot1 -GFP, whereas it did enhance its dimerization, suggesting that phot1 dimerization is independent of its phosphorylation. Förster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM) analysis revealed that the interaction between phot1-GFP and AtRem1.3-mCherry was enhanced along with increased time of BL treatment. However, the BL-dependent interaction was not obvious in plants co-expressing phot1 -GFP and AtRem1.3-mCherry, implicating that BL facilitated the translocation of functional phot1-GFP into AtRem1.3-labeled microdomains to activate phot-mediated signaling. Conversely, sterol depletion attenuated phot1-GFP dynamics, dimerization, and phosphorylation. Taken together, these results indicate that membrane microdomains act as an organizing platform essential for proper function of activated phot1 at the PM.

Item Type:Articles
Additional Information:This work is supported by the National Natural Science Foundation of China (31530084, 31270224) and the Program of Introducing Talents of Discipline to Universities (111 project, B13007).
Keywords:VA-TIRFM, blue light signaling, membrane microdomains, phot1, spatiotemporal dynamics.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Christie, Professor John
Authors: Xue, Y., Xing, J., Wan, Y., Lv, X., Fan, L., Zhang, Y., Song, K., Wang, L., Wang, X., Deng, X., Baluska, F., Christie, J. M., and Lin, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Molecular Plant
Publisher:Elsevier (Cell Press)
ISSN:1674-2052
ISSN (Online):1752-9867
Published Online:22 April 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Molecular Plant 11(6): 846-859
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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