Phage-inducible chromosomal islands are ubiquitous within the bacterial universe

Fillol-Salom, A., Martínez-Rubio, R., Abdulrahman, R. F., Chen, J., Davies, R. and Penades, J. R. (2018) Phage-inducible chromosomal islands are ubiquitous within the bacterial universe. ISME Journal, 12(9), pp. 2114-2128. (doi:10.1038/s41396-018-0156-3) (PMID:29875435) (PMCID:PMC6092414)

Fillol-Salom, A., Martínez-Rubio, R., Abdulrahman, R. F., Chen, J., Davies, R. and Penades, J. R. (2018) Phage-inducible chromosomal islands are ubiquitous within the bacterial universe. ISME Journal, 12(9), pp. 2114-2128. (doi:10.1038/s41396-018-0156-3) (PMID:29875435) (PMCID:PMC6092414)

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Abstract

Phage-inducible chromosomal islands (PICIs) are a recently discovered family of pathogenicity islands that contribute substantively to horizontal gene transfer, host adaptation and virulence in Gram-positive cocci. Here we report that similar elements also occur widely in Gram-negative bacteria. As with the PICIs from Gram-positive cocci, their uniqueness is defined by a constellation of features: unique and specific attachment sites, exclusive PICI genes, a phage-dependent mechanism of induction, conserved replication origin organization, convergent mechanisms of phage interference, and specific packaging of PICI DNA into phage-like infectious particles, resulting in very high transfer frequencies. We suggest that the PICIs represent two or more distinct lineages, have spread widely throughout the bacterial world, and have diverged much more slowly than their host organisms or their prophage cousins. Overall, these findings represent the discovery of a universal class of mobile genetic elements.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Penades, Professor Jose R and Davies, Dr Robert and Abdulrahman, Rezheen Fatah
Authors: Fillol-Salom, A., Martínez-Rubio, R., Abdulrahman, R. F., Chen, J., Davies, R., and Penades, J. R.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:ISME Journal
Publisher:Nature Publishing Group
ISSN:1751-7362
ISSN (Online):1751-7370
Published Online:06 June 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in ISME Journal 12(9): 2114-2128
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
660772Molecular biology of the PICIs, a novel and widespread family of mobile genetic elements involved in bacterial virulenceJose R PenadesMedical Research Council (MRC)MR/M003876/1RI INFECTION IMMUNITY & INFLAMMATION
693781Understanding a novel mechanim involving pathogenity islands in the transfer of unlinked chromosomal virulence genesJose R PenadesBiotechnology and Biological Sciences Research Council (BBSRC)BB/N002873/1RI INFECTION IMMUNITY & INFLAMMATION
727241Understanding bacterial host adaptation to combat infectious diseasesJose R PenadesWellcome Trust (WELLCOTR)201531/Z/16/ZRI INFECTION IMMUNITY & INFLAMMATION
687701DUT-SignalJose R PenadesEuropean Research Council (ERC)670932RI INFECTION IMMUNITY & INFLAMMATION