Stem cell-like populations and immunoregulatory molecules in periodontal granulation tissue

Apatzidou, D.A., Nile, C. , Bakopoulou, A., Konstantinidis, A. and Lappin, D.F. (2018) Stem cell-like populations and immunoregulatory molecules in periodontal granulation tissue. Journal of Periodontal Research, 53(4), pp. 610-621. (doi:10.1111/jre.12551) (PMID:29687448)

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Abstract

Background and Objectives: Determine the presence of mesenchymal stem cells (MSCs) in healthy periodontal tissue and periodontal granulation tissue (GT) and explore associations between immuno‐regulatory molecules and selected subgingival microorganisms. Material and Methods: Mesenchymal stem cells were isolated, propagated and characterised by flow cytometry from a region of healthy gingival tissue and inflamed GT of 10 systemically healthy non‐smokers with chronic periodontitis. Tissue levels of immunoregulatory molecules were determined by qPCR and Gingival Crevicular Fluid (GCF) levels by ELISA. Subgingival plaque levels of periodontal pathogens were determined by qPCR Results: Cells with MSC‐properties were isolated from both inflamed GT and healthy gingival (G) tissue. A pro‐inflammatory process predominated in GT which was partly reflected in GCF and putative periodontal pathogens were higher at diseased sites. However, there was no significant difference in surface levels of mesenchymal (CD90, CD73, CD146, CD271, STRO‐1), endothelial (CD105, CD106), hematopoietic (CD34, CD45) and embryonic (SSEA‐4) stem cell markers between MSCs isolated from GT and G tissue. Conclusion: Periodontal lesions, albeit inflamed, retain healing potential as inferred by the presence of MSC‐like cells with similar immunophenotypic characteristics to those found in healthy periodontal tissue. Therefore, there might be merits for healing in preserving sufficient GT in‐situ during periodontal surgery.

Item Type:Articles
Keywords:Periodontics.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lappin, Dr David and Nile, Dr Chris
Authors: Apatzidou, D.A., Nile, C., Bakopoulou, A., Konstantinidis, A., and Lappin, D.F.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Journal of Periodontal Research
Publisher:Wiley
ISSN:0022-3484
ISSN (Online):1600-0765
Published Online:23 April 2018
Copyright Holders:Copyright © 2018 John Wiley and Sons Ltd
First Published:First published in Journal of Periodontal Research 53(4): 610-621
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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