Control of cell behaviour through nanovibrational stimulation: nanokicking

Robertson, S. N. et al. (2018) Control of cell behaviour through nanovibrational stimulation: nanokicking. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 376(2120), 20170290. (doi:10.1098/rsta.2017.0290) (PMID:29661978) (PMCID:PMC5915650)

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Abstract

Mechanical signals are ubiquitous in our everyday life and the process of converting these mechanical signals into a biological signalling response is known as mechanotransduction. Our understanding of mechanotransduction, and its contribution to vital cellular responses, is a rapidly expanding field of research involving complex processes that are still not clearly understood. The use of mechanical vibration as a stimulus of mechanotransduction, including variation of frequency and amplitude, allows an alternative method to control specific cell behaviour without chemical stimulation (e.g. growth factors). Chemical-independent control of cell behaviour could be highly advantageous for fields including drug discovery and clinical tissue engineering. In this review, a novel technique is described based on nanoscale sinusoidal vibration. Using finite-element analysis in conjunction with laser interferometry, techniques that are used within the field of gravitational wave detection, optimization of apparatus design and calibration of vibration application have been performed. We further discuss the application of nanovibrational stimulation, or 'nanokicking', to eukaryotic and prokaryotic cells including the differentiation of mesenchymal stem cells towards an osteoblast cell lineage. Mechanotransductive mechanisms are discussed including mediation through the Rho-A kinase signalling pathway. Optimization of this technique was first performed in two-dimensional culture using a simple vibration platform with an optimal frequency and amplitude of 1 kHz and 22 nm. A novel bioreactor was developed to scale up cell production, with recent research demonstrating that mesenchymal stem cell differentiation can be efficiently triggered in soft gel constructs. This important step provides first evidence that clinically relevant (three-dimensional) volumes of osteoblasts can be produced for the purpose of bone grafting, without complex scaffolds and/or chemical induction. Initial findings have shown that nanovibrational stimulation can also reduce biofilm formation in a number of clinically relevant bacteria. This demonstrates additional utility of the bioreactor to investigate mechanotransduction in other fields of research.This article is part of a discussion meeting issue 'The promises of gravitational-wave astronomy'.

Item Type:Articles
Additional Information:Also funded by STFC (ST/N005406/1), Find A Better Way, SUPA, the Royal Society (RS), the Royal Society of Edinburgh (RSE), NHS Greater Glasgow & Clyde, and Linn Products Ltd; and the University of the West of Scotland, University of Glasgow and University of Strathclyde are gratefully acknowledged.
Keywords:Bacteria, gravitational waves, mechanotransduction, mesenchymal stem cells, nanokicking, nanovibrational stimulation.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Childs, Dr Peter and Dalby, Professor Matthew and Tsimbouri, Dr Penelope and Henriquez, Dr Fiona and DONNELLY, HANNAH and Campsie, Mr Paul and Williams, Dr Craig and MacKay, Dr William
Authors: Robertson, S. N., Campsie, P., Childs, P. G., Madsen, F., Donnelly, H., Henriquez, F. L., MacKay, W. G., Salmerón-Sánchez, M., Tsimbouri, M. P., Williams, C., Dalby, M. J., and Reid, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
College of Science and Engineering > School of Physics and Astronomy
Journal Name:Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
Publisher:Royal Society
ISSN:1364-503X
ISSN (Online):1471-2962
Published Online:16 April 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 376(2120):20170290
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.592

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
709981Development of NanoKick BioreactorMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/N012690/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
728381Rapid Bone Graft Synthesis Through Dual Piezoelectric/Nanomechaniocal StimulationMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/P00220X/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
691891Developing the NanoKick bioreactor to enable tissue engineered bone graft and use of metabolomics to identify bone specific drug candidatesMatthew DalbyEngineering and Physical Sciences Research Council (EPSRC)EP/N013905/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
722061Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - BIOMEDICAL ENGINEERING