Engineered coatings for titanium implants to present ultralow doses of BMP-7

Moulisova, V. , Al-Jarsha, M., Leal-Egaña, A., Connell, A., Naudi, K. B., Ayoub, A. F. , Dalby, M. J. and Salmeron-Sanchez, M. (2018) Engineered coatings for titanium implants to present ultralow doses of BMP-7. ACS Biomaterials Science and Engineering, 4(5), pp. 1812-1819. (doi:10.1021/acsbiomaterials.7b01037) (PMID:29862317) (PMCID:PMC5973637)

Moulisova, V. , Al-Jarsha, M., Leal-Egaña, A., Connell, A., Naudi, K. B., Ayoub, A. F. , Dalby, M. J. and Salmeron-Sanchez, M. (2018) Engineered coatings for titanium implants to present ultralow doses of BMP-7. ACS Biomaterials Science and Engineering, 4(5), pp. 1812-1819. (doi:10.1021/acsbiomaterials.7b01037) (PMID:29862317) (PMCID:PMC5973637)

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Abstract

The ongoing research to improve the clinical outcome of titanium implants has resulted in the implemetation of multiple approches to deliver osteogenic growth factors accelerating and sustaining osseointegration. Here we show the presentation of human bone morphogenetic protein 7 (BMP-7) adsorbed to titanium discs coated with poly(ethyl acrylate) (PEA). We have previously shown that PEA promotes fibronectin organization into nanonetworks exposing integrin- and growth-factor-binding domains, allowing a synergistic interaction at the integrin/growth factor receptor level. Here, titanium discs were coated with PEA and fibronectin and then decorated with ng/mL doses of BMP-7. Human mesenchymal stem cells were used to investigate cellular responses on these functionalized microenvironments. Cell adhesion, proliferation, and mineralization, as well as osteogenic markers expression (osteopontin and osteocalcin) revealed the ability of the system to be more potent in osteodifferentiation of the mesenchymal cells than combinations of titanium and BMP-7 in absence of PEA coatings. This work represents a novel strategy to improve the biological activity of titanium implants with BMP-7.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Moulisova, Dr Vladimira and Ayoub, Professor Ashraf and Connell, Andrew and Dalby, Professor Matthew
Authors: Moulisova, V., Al-Jarsha, M., Leal-Egaña, A., Connell, A., Naudi, K. B., Ayoub, A. F., Dalby, M. J., and Salmeron-Sanchez, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Science and Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:ACS Biomaterials Science and Engineering
Publisher:American Chemical Society
ISSN:2373-9878
ISSN (Online):2373-9878
Published Online:22 April 2018
Copyright Holders:Copyright © 2018 American Chemical Society
First Published:First published in ACS Biomaterials Science and Engineering 4:1812-1819
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
722061Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - BIOMEDICAL ENGINEERING
653731Synergistic microenvironments for non-union bone defectsMatthew DalbyMedical Research Council (MRC)MR/L022710/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
626901HEALINSYNERGY - Material-driven fibronectin fibrillogenesis to engineer synergistic growth factor microenvironmentsManuel Salmeron-SanchezEuropean Research Council (ERC)306990ENG - BIOMEDICAL ENGINEERING