The prismatic topography of Pinctada maxima shell retains stem cell multipotency and plasticity in vitro

Alakpa, E. V., Saeed, A. , Chung, P., Riehle, M. O. , Gadegaard, N. , Dalby, M. J. and Cusack, M. (2018) The prismatic topography of Pinctada maxima shell retains stem cell multipotency and plasticity in vitro. Advanced Biosystems, 2(6), 1800012. (doi:10.1002/adbi.201800012)

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Abstract

The shell of the bivalve mollusc Pinctada maxima is composed of the calcium carbonate polymorphs calcite and aragonite (nacre). Mother‐of‐pearl, or nacre, induces vertebrate cells to undergo osteogenesis and has good osteointegrative qualities in vivo. The calcite counterpart, however, is less researched in terms of the response of vertebrate cells. This study shows that isolation of calcite surface topography from the inherent chemistry allows viable long‐term culture of bone marrow derived mesenchymal stem cells (MSCs). Self‐renewal is evident from the increased gene expression of the self‐renewal markers CD63, CD166, and CD271 indicating that cells cultured on the calcite topography maintain their stem cell phenotype. MSCs also retain their multipotency and can undergo successful differentiation into osteoblasts and adipocytes. When directed to adipogenesis, MSCs cultured on prism replicas are more amenable to differentiation than MSCs cultured on tissue culture polystyrene indicating a higher degree of plasticity in MSCs growing on calcite P. maxima prismatic topography. The study highlights the potential of the calcite topography of P. maxima as a biomimetic design for supporting expansion of MSC populations in vitro, which is of fundamental importance if it meets the demands for autologous MSCs for therapeutic use.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Riehle, Dr Mathis and Saeed, Dr Anwer and Alakpa, Miss Enateri and Chung, Mr Peter and Cusack, Professor Maggie and Gadegaard, Professor Nikolaj
Authors: Alakpa, E. V., Saeed, A., Chung, P., Riehle, M. O., Gadegaard, N., Dalby, M. J., and Cusack, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Science and Engineering > School of Geographical and Earth Sciences
Journal Name:Advanced Biosystems
Publisher:Wiley
ISSN:2366-7478
ISSN (Online):2366-7478
Published Online:24 April 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Advanced Biosystems 2(6):1800012
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
609651Stem Cells metabolomics for bone therapies and tissue engineering.Maggie CusackMedical Research Council (MRC)MR/K011278/1SCHOOL OF GEOGRAPHICAL & EARTH SCIENCES