Vacuolar Protein Sorting 26C encodes an evolutionarily conserved large retromer subunit in eukaryotes that is important for root hair growth in Arabidopsis thaliana

Jha, S. G., Larson, E. R., Humble, J., Domozych, D. S., Barrington, D. S. and Tierney, M. L. (2018) Vacuolar Protein Sorting 26C encodes an evolutionarily conserved large retromer subunit in eukaryotes that is important for root hair growth in Arabidopsis thaliana. Plant Journal, 94(4), pp. 595-611. (doi: 10.1111/tpj.13880) (PMID:29495075)

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Abstract

The large retromer complex participates in diverse endosomal trafficking pathways and is essential for plant developmental programs, including cell polarity, programmed cell death and shoot gravitropism in Arabidopsis. Here we demonstrate that an evolutionarily conserved VPS26 protein (VPS26C; At1G48550) functions in a complex with VPS35A and VPS29 necessary for root hair growth in Arabidopsis. Bimolecular fluorescence complementation showed that VPS26C forms a complex with VPS35A in the presence of VPS29, and this is supported by genetic studies showing that vps29 and vps35a mutants exhibit altered root hair growth. Genetic analysis also demonstrated an interaction between a VPS26C trafficking pathway and one involving the SNARE VTI13. Phylogenetic analysis indicates that VPS26C, with the notable exception of grasses, has been maintained in the genomes of most major plant clades since its evolution at the base of eukaryotes. To test the model that VPS26C orthologs in animal and plant species share a conserved function, we generated transgenic lines expressing GFP fused with the VPS26C human ortholog (HsDSCR3) in a vps26c background. These studies illustrate that GFP‐HsDSCR3 is able to complement the vps26c root hair phenotype in Arabidopsis, indicating a deep conservation of cellular function for this large retromer subunit across plant and animal kingdoms.

Item Type:Articles
Additional Information:USDA-Hatch Grants VT-H02001 and VT-H02311 and funds from the Department of Plant Biology, University of Vermont, supported this research.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Larson, Dr Emily
Authors: Jha, S. G., Larson, E. R., Humble, J., Domozych, D. S., Barrington, D. S., and Tierney, M. L.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Plant Journal
Publisher:Wiley
ISSN:0960-7412
ISSN (Online):1365-313X
Published Online:06 April 2018

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