3D + time blood flow mapping using SPIM-microPIV in the developing zebrafish heart

Zickus, V. and Taylor, J. M. (2018) 3D + time blood flow mapping using SPIM-microPIV in the developing zebrafish heart. Biomedical Optics Express, 9(5), pp. 2418-2435. (doi:10.1364/BOE.9.002418) (PMID:29760998) (PMCID:PMC5946799)

Zickus, V. and Taylor, J. M. (2018) 3D + time blood flow mapping using SPIM-microPIV in the developing zebrafish heart. Biomedical Optics Express, 9(5), pp. 2418-2435. (doi:10.1364/BOE.9.002418) (PMID:29760998) (PMCID:PMC5946799)

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Abstract

We present SPIM-μPIV as a flow imaging system, capable of measuring in vivo flow information with 3D micron-scale resolution. Our system was validated using a phantom experiment consisting of a flow of beads in a 50 μm diameter FEP tube. Then, with the help of optical gating techniques, we obtained 3D + time flow fields throughout the full heartbeat in a ∼3 day old zebrafish larva using fluorescent red blood cells as tracer particles. From this we were able to recover 3D flow fields at 31 separate phases in the heartbeat. From our measurements of this specimen, we found the net pumped blood volume through the atrium to be 0.239 nL per beat. SPIM-μPIV enables high quality in vivo measurements of flow fields that will be valuable for studies of heart function and fluid-structure interaction in a range of small-animal models.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ži?kus, VYTAUTAS and Taylor, Dr Jonathan
Authors: Zickus, V., and Taylor, J. M.
College/School:College of Science and Engineering
College of Science and Engineering > School of Physics and Astronomy
Journal Name:Biomedical Optics Express
Publisher:Optical Society of America
ISSN:2156-7085
ISSN (Online):2156-7085
Published Online:27 April 2018
Copyright Holders:Copyright © 2018 Optical Society of America
First Published:First published in Biomedical Optics Express 9(5):2418-2435
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
678901EPSRC DTG 2014Mary Beth KneafseyEngineering and Physical Sciences Research Council (EPSRC)EP/M506539/1RSI - RESEARCH STRATEGY & INNOVATION
642161Development and optimisation of synchronised 3D in-vivo imaging of the embryonic and juvenile zebrafish heartJonathan TaylorBritish Heart Foundation (BHF)NH/14/2/31074S&E P&A - PHYSICS & ASTRONOMY
692111University of Glasgow Experimental Equipment ProposalAndrew HarveyEngineering and Physical Sciences Research Council (EPSRC)EP/M028135/1SCHOOL OF PHYSICS & ASTRONOMY
641351High speed flow field mapping in the embryonic zebrafish heartJonathan TaylorThe Royal Society (ROYSOC)RG130249S&E P&A - PHYSICS & ASTRONOMY