Urinary CE-MS peptide marker pattern for detection of solid tumors

Belczacka, I., Latosinska, A., Siwy, J., Metzger, J., Merseburger, A. S., Mischak, H., Vlahou, A., Frantzi, M. and Jankowski, V. (2018) Urinary CE-MS peptide marker pattern for detection of solid tumors. Scientific Reports, 8(1), 5227. (doi:10.1038/s41598-018-23585-y) (PMID:29588543)

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Abstract

Urinary profiling datasets, previously acquired by capillary electrophoresis coupled to mass-spectrometry were investigated to identify a general urinary marker pattern for detection of solid tumors by targeting common systemic events associated with tumor-related inflammation. A total of 2,055 urinary profiles were analyzed, derived from a) a cancer group of patients (n = 969) with bladder, prostate, and pancreatic cancers, renal cell carcinoma, and cholangiocarcinoma and b) a control group of patients with benign diseases (n = 556), inflammatory diseases (n = 199) and healthy individuals (n = 331). Statistical analysis was conducted in a discovery set of 676 cancer cases and 744 controls. 193 peptides differing at statistically significant levels between cases and controls were selected and combined to a multi-dimensional marker pattern using support vector machine algorithms. Independent validation in a set of 635 patients (293 cancer cases and 342 controls) showed an AUC of 0.82. Inclusion of age as independent variable, significantly increased the AUC value to 0.85. Among the identified peptides were mucins, fibrinogen and collagen fragments. Further studies are planned to assess the pattern value to monitor patients for tumor recurrence. In this proof-of-concept study, a general tumor marker pattern was developed to detect cancer based on shared biomarkers, likely indicative of cancer-related features.

Item Type:Articles
Additional Information:Tis work was supported by the Chromatin3D grant n°622934 (Chromatin3D-MSCA-ITN) project funded by the European Commission.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mischak, Professor Harald and Frantzi, Ms Maria
Authors: Belczacka, I., Latosinska, A., Siwy, J., Metzger, J., Merseburger, A. S., Mischak, H., Vlahou, A., Frantzi, M., and Jankowski, V.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Scientific Reports
Publisher:Nature Publishing Group
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Scientific Reports 8(1):5227
Publisher Policy:Reproduced under a Creative Commons License

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