Transcriptomic profiling of nematode parasites surviving vaccine exposure

Sallé, G. et al. (2018) Transcriptomic profiling of nematode parasites surviving vaccine exposure. International Journal for Parasitology, 48(5), pp. 395-402. (doi:10.1016/j.ijpara.2018.01.004) (PMID:29534987) (PMCID:PMC5909036)

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Abstract

Some nematode species are economically important parasites of livestock, while others are important human pathogens causing some of the most important neglected tropical diseases. In both humans and animals, anthelmintic drug administration is the main control strategy, but the emergence of drug-resistant worms has stimulated the development of alternative control approaches. Among these, vaccination is considered to be a sustainable and cost effective strategy. Currently, Barbervax® for the ruminant strongylid Haemonchus contortus is the only registered subunit vaccine for a nematode parasite, although a vaccine for the human hookworm Necator americanus is undergoing clinical trials (HOOKVAC consortium). As both these vaccines comprise a limited number of proteins, there is potential for selection of nematodes with altered sequences or expression of the vaccine antigens. Here we compared the transcriptome of H. contortus populations from sheep vaccinated with Barbervax® with worms from control animals. Barbervax® antigens are native integral membrane proteins isolated from the brush border of the intestinal cells of the adult parasite and many of those are proteases. Our findings provide no evidence for changes in expression of genes encoding Barbervax® antigens in the surviving parasite populations. However, surviving parasites from vaccinated animals showed increased expression of other proteases and regulators of lysosome trafficking, and displayed up-regulated lipid storage and defecation abilities that may have circumvented the effect of the vaccine. Implications for other potential vaccines for human and veterinary nematodes are discussed.

Item Type:Articles
Keywords:Barbervax, Haemonchus contortus, nematode, protease, transcriptome, vaccine.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen and Laing, Dr Rosalind and Hanks, Eve and Maitland, Ms Kirsty and Britton, Dr Collette
Authors: Sallé, G., Laing, R., Cotton, J. A., Maitland, K., Martinelli, A., Holroyd, N., Tracey, A., Berriman, M., Smith, W. D., Newlands, G. F.J., Hanks, E., Devaney, E., and Britton, C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:International Journal for Parasitology
Publisher:Elsevier
ISSN:0020-7519
ISSN (Online):1879-0135
Published Online:10 March 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in International Journal for Parasitology 48(5):395-402
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
636401The BUG consortium Building Upon the Genome: using H. contortus genomic resources to develop novel interventions to control endemic GI parasitesEileen DevaneyBiotechnology and Biological Sciences Research Council (BBSRC)BB/M003949/1RI BIODIVERSITY ANIMAL HEALTH & COMPMED