Oximetry using multispectral imaging: theory and application

MacKenzie, L. E. and Harvey, A. R. (2018) Oximetry using multispectral imaging: theory and application. Journal of Optics, 20(6), 063501. (doi:10.1088/2040-8986/aab74c)

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Abstract

Multispectral imaging (MSI) is a technique for measurement of blood oxygen saturation in vivo that can be applied using various imaging modalities to provide new insights into physiology and disease development. This tutorial aims to provide a thorough introduction to the theory and application of MSI oximetry for researchers new to the field, whilst also providing detailed information for more experienced researchers. The optical theory underlying two-wavelength oximetry, three-wavelength oximetry, pulse oximetry, and multispectral oximetry algorithms are described in detail. The varied challenges of applying MSI oximetry to in vivo applications are outlined and discussed, covering: the optical properties of blood and tissue, optical paths in blood vessels, tissue auto-fluorescence, oxygen diffusion, and common oximetry artefacts. Essential image processing techniques for MSI are discussed, in particular, image acquisition, image registration strategies, and blood vessel line profile fitting. Calibration and validation strategies for MSI are discussed, including comparison techniques, physiological interventions, and phantoms. The optical principles and unique imaging capabilities of various cutting-edge MSI oximetry techniques are discussed, including photoacoustic imaging, spectroscopic optical coherence tomography, and snapshot MSI.

Item Type:Articles
Additional Information:The University of Glasgow Sensors Initiative provided funding for Lewis MacKenzie’s PhD studentship, between 2012 and 2016.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Harvey, Professor Andy and MACKENZIE, LEWIS
Authors: MacKenzie, L. E., and Harvey, A. R.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Journal of Optics
Publisher:IOP Publishing
ISSN:2040-8978
ISSN (Online):2040-8986
Published Online:16 March 2018
Copyright Holders:Copyright © 2018 IOP Publishing Ltd
First Published:First published in Journal of Optics 20(6): 063501
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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