Plasmodium gametocytes display homing and vascular transmigration in the host bone marrow

De Niz, M. et al. (2018) Plasmodium gametocytes display homing and vascular transmigration in the host bone marrow. Science Advances, 4(5), eaat3775. (doi:10.1126/sciadv.aat3775) (PMID:29806032) (PMCID:PMC5966192)

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Abstract

Transmission of Plasmodium parasites to the mosquito requires the formation and development of gametocytes. Studies in infected humans have shown that only the most mature forms of Plasmodium falciparum gametocytes are present in circulation, whereas immature forms accumulate in the hematopoietic environment of the bone marrow. We used the rodent model Plasmodium berghei to study gametocyte behavior through time under physiological conditions. Intravital microscopy demonstrated preferential homing of early gametocyte forms across the intact vascular barrier of the bone marrow and the spleen early during infection and subsequent development in the extravascular environment. During the acute phase of infection, we observed vascular leakage resulting in further parasite accumulation in this environment. Mature gametocytes showed high deformability and were found entering and exiting the intact vascular barrier. We suggest that extravascular gametocyte localization and mobility are essential for gametocytogenesis and transmission of Plasmodium to the mosquito.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hughes, Dr Katie and Waters, Professor Andrew and Ngotho, Dr Priscilla and Marti, Professor Matthias and Brancucci, Dr Nicolas and De Niz, Dr Mariana
Authors: De Niz, M., Meibalan, E., Mejia, P., Ma, S., Brancucci, N. M.B., Agop-Nersesian, C., Mandt, R., Ngotho, P., Hughes, K. R., Waters, A. P., Huttenhower, C., Mitchell, J. R., Martinelli, R., Frischknecht, F., Seydel, K. B., Taylor, T., Milner, D., Heussler, V. T., and Marti, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Science Advances
Publisher:American Association for the Advancement of Science
ISSN:2375-2548
ISSN (Online):2375-2548
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Science Advances 4(5): eaat3775
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
690321Gene expression in Plasmodium parasites: the molecular mechanics of gametocytogenesis (and variant transcription of genes)Andrew WatersWellcome Trust (WELLCOTR)107046/Z/15/ZIII - PARASITOLOGY
371799The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/Z & AIII - PARASITOLOGY