Rattigan, K. M., Pountain, A. W. , Regnault, C., Achcar, F. , Vincent, I. M. , Goodyear, C. S. and Barrett, M. P. (2018) Metabolomic profiling of macrophages determines the discrete metabolomic signature and metabolomic interactome triggered by polarising immune stimuli. PLoS ONE, 13(3), e0194126. (doi: 10.1371/journal.pone.0194126) (PMID:29538444) (PMCID:PMC5851634)
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Abstract
Priming and activating immune stimuli have profound effects on macrophages, however, studies generally evaluate stimuli in isolation rather than in combination. In this study we have investigated the effects of pro-inflammatory and anti-inflammatory stimuli either alone or in combination on macrophage metabolism. These stimuli include host factors such as IFNγ and ovalbumin-immunoglobulin immune complexes, or pathogen factors such as LPS. Untargeted LC-MS based metabolomics provided an in-depth profile of the macrophage metabolome, and revealed specific changes in metabolite abundance upon either individual stimuli or combined stimuli. Here, by factoring in an interaction term in the linear model, we define the metabolome interactome. This approach allowed us to determine whether stimuli interact in a synergistic or antagonistic manner. In conclusion this study demonstrates a robust approach to interrogate immune-metabolism, especially systems that model host-pathogen interactions.
Item Type: | Articles |
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Keywords: | biology and life sciences, medicine and health sciences, physical sciences. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Rattigan, Dr Kevin and Vincent, Dr Isabel and Regnault, Mr Clement and Achcar, Dr Fiona and Goodyear, Professor Carl and Pountain, Andrew and Barrett, Professor Michael |
Creator Roles: | Rattigan, K. M.Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review and editing Pountain, A. W.Formal analysis, Methodology Regnault, C.Methodology Achcar, F.Formal analysis, Methodology Vincent, I. M.Formal analysis Goodyear, C. S.Conceptualization, Funding acquisition, Investigation, Methodology, Supervision, Writing – review and editing Barrett, M. P.Conceptualization, Funding acquisition, Investigation, Methodology, Resources, Supervision, Writing – original draft, Writing – review and editing |
Authors: | Rattigan, K. M., Pountain, A. W., Regnault, C., Achcar, F., Vincent, I. M., Goodyear, C. S., and Barrett, M. P. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | PLoS ONE |
Publisher: | Public Library of Science |
ISSN: | 1932-6203 |
ISSN (Online): | 1932-6203 |
Copyright Holders: | Copyright © 2018 Rattigan et al. |
First Published: | First published in PLoS ONE 1393):e0194126 |
Publisher Policy: | Reproduced under a Creative Commons License |
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