Mclean, K. et al. (2018) Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry. Talanta, 182, pp. 164-170. (doi: 10.1016/j.talanta.2018.01.055) (PMID:29501136)
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Abstract
Whole cell MALDI is regularly used for the identification of bacteria to species level in clinical Microbiology laboratories. However, there remains a need to rapidly characterize and differentiate isolates below the species level to support outbreak management. We describe the implementation of a modified preparative approach for MALDI-MS combined with a custom analytical computational pipeline as a rapid procedure for subtyping Shigatoxigenic E. coli (STEC) and accurately identifying strain-specifying biomarkers. The technique was able to differentiate E. coli O157:H7 from other STEC. Within O157 serotype O157:H7 isolates were readily distinguishable from Sorbitol Fermenting O157 isolates. Overall, nine homogeneous groups of isolates were distinguished, each exhibiting distinct profiles of defining mass spectra features. This offers a robust analytical tool useable in reference/diagnostic public health scenarios.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Kerr, Mrs Erin |
Authors: | Mclean, K., Palarea-Albaladejo, J., Currie, C. G., Imrie, L. H.J., Manson, E. D.T., Fraser-Pitt, D., Wright, F., Alexander, C. J., Pollock, K. G.J., Allison, L., Hanson, M., and Smith, D. G.E. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Talanta |
Publisher: | Elsevier |
ISSN: | 0039-9140 |
ISSN (Online): | 1873-3573 |
Published Online: | 20 February 2018 |
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