Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach

Bilić, P., Guillemin, N., Kovačević, A., Ljubić, B. B., Jović, I., Galan, A., Eckersall, P. D. , Burchmore, R. and Mrljak, V. (2018) Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach. Journal of Proteomics, 179, pp. 110-121. (doi: 10.1016/j.jprot.2018.03.007) (PMID:29530679)

Abstract

Idiopathic dilated cardiomyopathy (iDCM) is a primary myocardial disorder with an unknown aetiology, characterized by reduced contractility and ventricular dilation of the left or both ventricles. Naturally occurring canine iDCM was used herein to identify serum proteomic signature of the disease compared to the healthy state, providing an insight into underlying mechanisms and revealing proteins with biomarker potential. To achieve this, we used high-throughput label-based quantitative LC-MS/MS proteomics approach and bioinformatics analysis of the in silico inferred interactome protein network created from the initial list of differential proteins. To complement the proteomic analysis, serum biochemical parameters and levels of know biomarkers of cardiac function were measured. Several proteins with biomarker potential were identified, such as inter-alpha-trypsin inhibitor heavy chain H4, microfibril-associated glycoprotein 4 and apolipoprotein A-IV, which were validated using an independent method (Western blotting) and showed high specificity and sensitivity according to the receiver operating characteristic curve analysis. Bioinformatics analysis revealed involvement of different pathways in iDCM, such as complement cascade activation, lipoprotein particles dynamics, elastic fibre formation, GPCR signalling and respiratory electron transport chain.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Eckersall, Professor David and Burchmore, Dr Richard
• Authors: Bilić, P., Guillemin, N., Kovačević, A., Ljubić, B. B., Jović, I., Galan, A., Eckersall, P. D., Burchmore, R., and Mrljak, V.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Journal of Proteomics
• Publisher: Elsevier
• ISSN: 1874-3919
• ISSN (Online): 1874-3919
• Published Online: 09 March 2018
• Copyright Holders: Copyright © 2018 Elsevier B.V.
• First Published: First published in Journal of Proteomics 179: 110-121
• Publisher Policy: Reproduced in accordance with the publisher copyright policy