Pearson, M.S., McManus, D.P., Smyth, D.J. , Jones, M.K., Sykes, A.M. and Loukas, A. (2007) Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni. Parasitology, 134(14), pp. 2001-2008. (doi: 10.1017/S0031182007003393) (PMID:17714602) (PMCID:PMC2753299)
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Abstract
A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven transmembrane (7TM) receptors from vertebrates and invertebrates, including the human ee3 receptor – a heptahelical protein implicated in neuronal signalling. Phylogenetic analysis of this novel family showed that the Sm-7TM ORF formed a clade with exclusively invertebrate sequences. Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was funded by a project grant (A.L.) and a block grant (D.P.M.) from the National Health and Medical Research Council of Australia (NHMRC). M.S.P. was supported by an Australian Postgraduate Award. A.L. was supported by a Career Development Award from the NHMRC. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Smyth, Dr Danielle |
| Authors: | Pearson, M.S., McManus, D.P., Smyth, D.J., Jones, M.K., Sykes, A.M., and Loukas, A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Parasitology |
| Publisher: | Cambridge University Press |
| ISSN: | 0031-1820 |
| ISSN (Online): | 1469-8161 |
| Published Online: | 23 August 2007 |
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