Mancini, S. J. and Salt, I. P. (2018) Investigating the role of AMPK in inflammation. In: Neumann, D. and Viollet, B. (eds.) AMPK: Methods and Protocols. Series: Methods in Molecular Biology, 1732. Humana Press, pp. 307-319. ISBN 9781493975976 (doi: 10.1007/978-1-4939-7598-3_20)
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Abstract
In addition to the well-characterized role of AMPK in the regulation of nutrient metabolism, it is increasingly clear that AMPK activation has multiple actions on inflammatory signalling. Here we describe methods to identify effects of AMPK activity on pro-inflammatory signalling, specifically (1) the nuclear localization of the key inflammatory mediators nuclear factor-κB (NFκB) and phosphorylated c-Jun N-terminal kinase (JNK), (2) preparation of conditioned medium to analyze the secretion of cytokines/chemokines, and (3) the pro-inflammatory adhesion of leukocytes to cultured cells.
Item Type: | Book Sections |
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Status: | Published |
Glasgow Author(s) Enlighten ID: | Mancini, Dr Sarah and Salt, Dr Ian |
Authors: | Mancini, S. J., and Salt, I. P. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Publisher: | Humana Press |
ISSN: | 1064-3745 |
ISBN: | 9781493975976 |
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