Testicular cell selective ablation using diphtheria toxin receptor transgenic mice

Rebourcet, D., Darbey, A., Curley, M., O'Shaughnessy, P. and Smith, L. B. (2018) Testicular cell selective ablation using diphtheria toxin receptor transgenic mice. In: Alves, M. G. and Oliveira, P. F. (eds.) Steroli Cells. Series: Methods in molecular biology (1748). Humana Press: New York, NY, pp. 203-228. ISBN 9781493976973 (doi: 10.1007/978-1-4939-7698-0_15)

Abstract

Testis development and function is regulated by intricate cell-cell cross talk. Characterization of the mechanisms underpinning this has been derived through a wide variety of approaches including pharmacological manipulation, transgenics, and cell-specific ablation of populations. The removal of all or a proportion of a specific cell type has been achieved through a variety of approaches. In this paper, we detail a combined transgenic and pharmacological approach to ablate the Sertoli or germ cell populations using diphtheria toxin in mice. We describe the key steps in generation, validation, and use of the models and also describe the caveats and cautions necessary. We also provide a detailed description of the methodology applied to characterize testis development and function in models of postnatal Sertoli or germ cell ablation.

• Item Type: Book Sections
• Keywords: Cre/lox, diphtheria, mouse, sertoli cells, testis, transgenic.
• Status: Published
• Glasgow Author(s) Enlighten ID: O'Shaughnessy, Professor Peter
• Authors: Rebourcet, D., Darbey, A., Curley, M., O'Shaughnessy, P., and Smith, L. B.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Publisher: Humana Press
• ISBN: 9781493976973