A Novel Multi-pad Paper Plate (MP3) Based Assays for Rapid Animal Disease Diagnostics

Busin, V., Burgess, S. and Shu, W. (2016) A Novel Multi-pad Paper Plate (MP3) Based Assays for Rapid Animal Disease Diagnostics. In: 30th Eurosensors Conference, EUROSENSORS 2016, Budapest, Hungary, 4-7 Sept 2016, pp. 1418-1421.

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Abstract

This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.

Item Type:Conference Proceedings
Additional Information:Published in Procedia Engineering, 168:1418-1421
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Busin, Valentina
Authors: Busin, V., Burgess, S., and Shu, W.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Procedia Engineering
Publisher:Elsevier
ISSN:1877-7058
ISSN (Online):1877-7058
Published Online:04 January 2017
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Procedia Engineering 168:1418-1421
Publisher Policy:Reproduced under a Creative Commons License

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