A defined medium for Leishmania culture allows definition of essential amino acids

Nayak, A., Akpunarlieva, S., Barrett, M. and Burchmore, R. (2018) A defined medium for Leishmania culture allows definition of essential amino acids. Experimental Parasitology, 185, pp. 39-52. (doi:10.1016/j.exppara.2018.01.009) (PMID:29326050)

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Abstract

Axenic culture of Leishmania is generally performed in rich, serum-supplemented media which sustain robust growth over multiple passages. The use of such undefined media, however, obscures proteomic analyses and confounds the study of metabolism. We have established a simple, defined culture medium that supports the sustained growth of promastigotes over multiple passages and which yields parasites that have similar infectivity to macrophages to parasites grown in a conventional semi-defined medium. We have exploited this medium to investigate the amino acid requirements of promastigotes in culture and have found that phenylalanine, tryptophan, arginine, leucine, lysine and valine are essential for viability in culture. Most of the 20 proteogenic amino acids promote growth of Leishmania promastigotes, with the exception of alanine, asparagine, and glycine. This defined medium will be useful for further studies of promastigote substrate requirements, and will facilitate future proteomic and metabolomic analyses.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Akpunarlieva, Miss Snezhana and Nayak, Archana and Barrett, Professor Michael
Authors: Nayak, A., Akpunarlieva, S., Barrett, M., and Burchmore, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Experimental Parasitology
Publisher:Elsevier
ISSN:0014-4894
ISSN (Online):1090-2449
Published Online:08 January 2018
Copyright Holders:Copyright © 2018 Elsevier Inc.
First Published:First published in Experimental Parasitology 185: 39-52
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
470561DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING