Cloning an Aspergillus nidulans developmental gene by transformation

Johnstone, I.L., Hughes, S.G. and Clutterbuck, A.J. (1985) Cloning an Aspergillus nidulans developmental gene by transformation. EMBO Journal, 4(5), pp. 1307-1311. (PMID:3891328) (PMCID:PMC554341)

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We have developed a transformation system for Aspergillus nidulans giving a frequency of transformation high enough to screen a gene bank from which we were able to isolate and clone the A. nidulans developmental gene brlA by visual selection. The vector contains the selective marker argB+, and with it a frequency of transformation of 500 stable transformants/micrograms plasmid DNA can regularly be achieved. The evidence suggests that transformation is by integration but spontaneous excision of integrated plasmids is apparently frequent enough to allow the recovery of transforming plasmids in Escherichia coli.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Johnstone, Professor Iain and Clutterbuck, Dr Arthur
Authors: Johnstone, I.L., Hughes, S.G., and Clutterbuck, A.J.
Subjects:Q Science > QH Natural history > QH426 Genetics
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:EMBO Journal
Publisher:IRL Press
ISSN (Online):1460-2075

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