Herbert, J. A., Mitchell, A. M., Ritchie, R., Ma, J., Ross-Hutchinson, K. and Mitchell, T. J. (2018) Expression of the lux genes in Streptococcus pneumoniae modulates pilus expression and virulence. PLoS ONE, 13(1), e0189426. (doi: 10.1371/journal.pone.0189426) (PMID:29342160) (PMCID:PMC5771582)
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Abstract
Bioluminescence has been harnessed for use in bacterial reporter systems and for in vivo imaging of infection in animal models. Strain Xen35, a bioluminescent derivative of Streptococcus pneumoniae serotype 4 strain TIGR4 was previously constructed for use for in vivo imaging of infections in animal models. We have shown that strain Xen35 is less virulent than its parent TIGR4 and that this is associated with the expression of the genes for bioluminescence. The expression of the luxA-E genes in the pneumococcus reduces virulence and down regulates the expression of the pneumococcal pilus.
Item Type: | Articles |
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Additional Information: | Work was funded using a PhD studentship for Dr Jenny Herbert from the Biotechnology and Biological Sciences Research Council (BBSRC). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Ross, Dr Kirsty and Mitchell, Dr Andrea and Mitchell, Professor Timothy and Ritchie, Mr Ryan and Ma, Dr Jiangtao |
Creator Roles: | Mitchell, A. M.Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Writing – review and editing Ritchie, R.Investigation, Writing – review and editing Ma, J.Investigation, Methodology, Writing – review and editing Ross-Hutchinson, K.Investigation, Writing – review and editing Mitchell, T. J.Conceptualization, Funding acquisition, Project administration, Supervision, Visualization, Writing – original draft, Writing – review and editing |
Authors: | Herbert, J. A., Mitchell, A. M., Ritchie, R., Ma, J., Ross-Hutchinson, K., and Mitchell, T. J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | PLoS ONE |
Publisher: | Public Library of Science |
ISSN: | 1932-6203 |
ISSN (Online): | 1932-6203 |
Copyright Holders: | Copyright © 2018 Herbert et al. |
First Published: | First published in PLoS ONE 13(1): e0189426 |
Publisher Policy: | Reproduced under a Creative Commons License |
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