Expression of the lux genes in Streptococcus pneumoniae modulates pilus expression and virulence

Herbert, J. A., Mitchell, A. M., Ritchie, R., Ma, J., Ross-Hutchinson, K. and Mitchell, T. J. (2018) Expression of the lux genes in Streptococcus pneumoniae modulates pilus expression and virulence. PLoS ONE, 13(1), e0189426. (doi: 10.1371/journal.pone.0189426) (PMID:29342160) (PMCID:PMC5771582)

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Abstract

Bioluminescence has been harnessed for use in bacterial reporter systems and for in vivo imaging of infection in animal models. Strain Xen35, a bioluminescent derivative of Streptococcus pneumoniae serotype 4 strain TIGR4 was previously constructed for use for in vivo imaging of infections in animal models. We have shown that strain Xen35 is less virulent than its parent TIGR4 and that this is associated with the expression of the genes for bioluminescence. The expression of the luxA-E genes in the pneumococcus reduces virulence and down regulates the expression of the pneumococcal pilus.

Item Type:Articles
Additional Information:Work was funded using a PhD studentship for Dr Jenny Herbert from the Biotechnology and Biological Sciences Research Council (BBSRC).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ross, Dr Kirsty and Mitchell, Dr Andrea and Mitchell, Professor Timothy and Ritchie, Mr Ryan and Ma, Dr Jiangtao
Creator Roles:
Mitchell, A. M.Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Writing – review and editing
Ritchie, R.Investigation, Writing – review and editing
Ma, J.Investigation, Methodology, Writing – review and editing
Ross-Hutchinson, K.Investigation, Writing – review and editing
Mitchell, T. J.Conceptualization, Funding acquisition, Project administration, Supervision, Visualization, Writing – original draft, Writing – review and editing
Authors: Herbert, J. A., Mitchell, A. M., Ritchie, R., Ma, J., Ross-Hutchinson, K., and Mitchell, T. J.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2018 Herbert et al.
First Published:First published in PLoS ONE 13(1): e0189426
Publisher Policy:Reproduced under a Creative Commons License

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